A kind of silk fibroin antibacterial dressing loaded with silver on one side and its preparation method and application

A technology of silk fibroin and solution, applied in the direction of resisting vector-borne diseases, bandages, absorbent pads, etc., can solve the problem of cell toxicity, achieve huge application potential, expand the treatment window, and reduce the effect of cytotoxicity

Active Publication Date: 2022-05-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no consistent conclusion on the effect of silver-containing dressings on wound healing. The possible reason is that silver is a double-edged sword, which has antibacterial properties and is also toxic to cells.

Method used

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  • A kind of silk fibroin antibacterial dressing loaded with silver on one side and its preparation method and application
  • A kind of silk fibroin antibacterial dressing loaded with silver on one side and its preparation method and application
  • A kind of silk fibroin antibacterial dressing loaded with silver on one side and its preparation method and application

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preparation example Construction

[0062] The second aspect of the present invention provides the preparation method of the silver-loaded silk fibroin antibacterial dressing on one side described in the first aspect, the preparation method comprising the following steps:

[0063] Dissolve sodium carbonate in boiled distilled water, add shredded cocoon shells and stir until degummed, wash and soak the degummed silk fibroin in distilled water to remove residual sodium carbonate on the surface, and dry the cleaned silk fibroin completely at room temperature Dissolve calcium chloride in formic acid to form a formic acid-calcium chloride mixed solution, then add silk fibroin to the above mixed solution and stir until the silk fibroin is completely dissolved to form a silk fibroin solution; add silver nitrate aqueous solution to silk Silver-silk fibroin (Ag-SF) solution is formed in the protein solution, and the silver-silk fibroin solution is evenly dispersed on the mold and volatilized. Antibacterial dressings of s...

Embodiment 1

[0076] Experimental materials and methods:

[0077] (1) Preparation and characterization of SF film and Ag-SF / SF antibacterial dressing

[0078] 1. Preparation of Silk Fibroin Solution

[0079] After heating 1.2L of distilled water to boiling, add 2.55g of sodium carbonate to dissolve, then add 3g of shredded cocoon shells, and continue to cook for 40min until degumming. The degummed silk fibroin was rinsed three times with distilled water, soaked overnight in 1L of distilled water to remove residual sodium carbonate on the surface, and completely dried at room temperature after taking it out. Dissolve and weigh 0.2-1g calcium chloride, dissolve it in 10-15mL formic acid (mass fraction 85-90%), add 1-2g degummed silk fibroin into the above solution and stir until completely dissolved for further use.

[0080] 2. Preparation of SF film and Ag-SF / SF antibacterial dressing

[0081] The Petri dish served as a mold for the preparation of the membrane. First, prepare 0.03-0.24 m...

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Abstract

The invention specifically relates to a silk fibroin antibacterial dressing loaded with silver on one side, a preparation method and application thereof. Silver has good antibacterial properties and can be added to antibacterial agents on wounds, but studies have shown that silver may also have cytotoxicity and reduce wound cell viability. In view of the above technical problems, the present invention provides a silk fibroin antibacterial dressing with unilateral loading and in-situ generation of AgCl particles. The antibacterial dressing includes a silver-loaded layer and a silk fibroin layer. Provide a good growth environment, at the same time, the silver-loaded layer continuously releases Ag through the buffering effect of the silk fibroin layer + , while reducing cytotoxicity to the wound surface, it also acts as an antibacterial agent. The antibacterial dressing has the advantages of simple preparation process and promotion of wound healing.

Description

technical field [0001] The invention belongs to the technical field of antibacterial wound dressings, and in particular relates to a silver-loaded silk fibroin antibacterial dressing on one side, a preparation method of the antibacterial dressing and an application of the antibacterial dressing as a wound dressing. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Silk fibroin (SF) is a natural protein extracted from silk. It has the ability to promote cell migration, proliferation, angiogenesis and re-epithelialization, and has a similar structure to extracellular matrix, so it is widely used in wound dressings. Dressings using silk fibroin as a scaffold material usually h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/18A61L15/46A61L15/44
CPCA61L15/32A61L15/18A61L15/46A61L15/44A61L2300/104A61L2300/404A61L2300/412C08L89/00Y02A50/30
Inventor 葛少华邵金龙马保金崔雅婷商玲玲
Owner SHANDONG UNIV
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