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Stem cell culture device for biological products

A technology for stem cell culture and biological products, applied in stem cell culture devices for biological products, and in the field of stem cell culture, can solve problems affecting stem cell culture and achieve the effect of easy installation and disassembly

Pending Publication Date: 2021-12-21
西藏鹰山科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is: in order to solve the problem in the prior art that adding culture fluid to the culture vessel through the pipeline will bring the bacteria in the pipeline into the culture vessel, thus affecting the cultivation of stem cells, the present invention provides a method for For the stem cell culture device of biological products, pass the feeding conduit into the transition port, then pour the culture solution into the feeding conduit to add the culture solution to the culture vessel, then pull the feeding conduit out of the transition port to clean it, and then turn the seal The blockage prevents the transition port from connecting with the feed port, which can prevent bacteria from entering the culture vessel through the transition port, and the cleaned feed conduit has fewer bacteria, which can reduce the bacteria entering the culture vessel, which is more conducive to stem cells. cultivation

Method used

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  • Stem cell culture device for biological products
  • Stem cell culture device for biological products
  • Stem cell culture device for biological products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Such as figure 1 As shown, the present embodiment provides a stem cell culture device for biological products, including an incubator 1, a culture vessel 5 is installed in the incubator 1, a case cover 9 is installed on the top surface of the incubator 1, and the case cover 9 is opened. There is a feeding port 12 communicated with the incubator 1, and a sealing plug 11 that can close the feeding port 12 is installed in the feeding port 12, and a transition port 111 that can communicate with the feeding port 12 is provided in the sealing plug 11, and the transition port The feed conduit 10 extending to the culture vessel 5 can be installed in the 111, and the plug 11 can be rotated in the feed port 12.

[0036]Working principle: when it is necessary to cultivate stem cells through the incubator, put the culture solution and the like in the culture vessel 5 in a conventional way, and then cover the box cover 9 on the top surface of the incubator 1, when it is necessary to...

Embodiment 2

[0041] Such as Figure 1-Figure 7 As shown, on the basis of the above-mentioned embodiment 1, the present embodiment provides a preference, the feed port 12 includes an upper feed nozzle 123, an installation groove 122 and a lower feed nozzle 121 connected sequentially from top to bottom, and the installation The groove 122 is spherical, the sealing plug 11 is installed in the installation groove 122, the upper feed nozzle 123 and the lower feed nozzle 121 are arranged obliquely, and the center line of the upper feed nozzle 123 and the center line of the lower feed nozzle 121 are on the same straight line On the top, the inner diameter of the upper feed nozzle 123 gradually decreases from top to bottom, and the inner diameter of the lower feed nozzle 121 gradually increases from top to bottom.

[0042] In this embodiment: when it is necessary to increase the culture solution in different directions in the culture vessel 5, the feed conduit 10 can be swung, and the feed conduit...

Embodiment 3

[0045] Such as figure 1 As shown, on the basis of the above-mentioned embodiment 1, the present embodiment provides a preferred structure that is more conducive to the cultivation of stem cells: a partition 3 is installed at the bottom of the incubator 1, the top of the partition 3 is a culture chamber 14, and the bottom of the partition 3 is a culture chamber 14. For the heating chamber 15 , the culture vessel 5 is installed in the culture chamber 14 , and the heating block 2 is installed in the heating chamber 15 . By heating the conventional heating block 2 , the heat emitted by the heating block 2 can diffuse into the culture vessel through the partition 3 , thereby providing a more suitable temperature environment for the stem cells in the culture vessel 5 .

[0046] Further, the inner wall of the incubator 1 is provided with a rotating tank 101, the culture vessel 5 is installed in the rotation tank 101, and the bottom surface of the culture vessel 5 is equipped with a r...

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Abstract

The invention discloses a stem cell culture device for biological products, relates to the technical field of stem cell culture, and aims to solve the problem that in the prior art, when a culture solution is added into a culture vessel through a pipeline, bacteria in the pipeline can be brought into a culture vessel, so that stem cell culture is affected. The stem cell culture device comprises a culture box, wherein a culture vessel is mounted in the culture box, a box cover is mounted on the top surface of the culture box, a feeding port communicating with the culture box is formed in the box cover, a sealing plug capable of sealing the feeding port is mounted in the feeding port, and a transition port communicating with the feeding port is formed in the sealing plug. A feeding guide pipe extending into the culture vessel can be mounted in the transition port, and the sealing plug can rotate in the feeding port. According to the technical scheme, bacteria entering the culture vessel can be reduced, and culture of stem cells is further facilitated.

Description

technical field [0001] The present invention relates to the technical field of stem cell culture, more specifically to the technical field of a stem cell culture device used for biological products. Background technique [0002] Biological products refer to the preparation of biological materials such as microorganisms, cells, tissues and liquids of various animal and human origins obtained by ordinary or genetic engineering, cell engineering, protein engineering, fermentation engineering, etc., and are used for the prevention of human diseases , medicines for treatment and diagnosis; stem cells are the origin cells, which are a kind of primitive undifferentiated cells with multi-directional differentiation potential and self-replication ability, and are the original cells that form various tissues and organs of mammals. Stem cells can be cultivated Obtaining and cultivating stem cells requires corresponding culture devices. [0003] The stem cell culture device in the prio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/34C12M1/26C12M1/22C12M1/08C12M1/02
CPCC12M23/02C12M23/10C12M23/38C12M23/34C12M27/02C12M29/06C12M33/04C12M41/22
Inventor 樊华张智博张沛薛润枞
Owner 西藏鹰山科技有限公司
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