Large-scale continuous culture method of gambierdiscus

Abstract

The invention relates to a large-scale continuous culture method of gambierdiscus. The large-scale continuous culture method comprises the following steps that 1) enlarged culture of an gambierdiscus algae species is carried out, specifically, the algae species is transferred to a slant cell culture flask, the algae species is horizontally placed for culture until an exponential phase, then transferring is carried out, and the procedure is repeated to keep the algae species at the highest productivity level; 2) photobioreactor culture of the gambierdiscus, specifically, exponential-phase algae liquid is poured into a barrel containing a liquid culture medium, the algae liquid is uniformly mixed in the barrel, and finally the algae liquid in the barrel is transferred into a photobioreactor for culture; the photobioreactor adopts a vertical drawer type structure; and 3) semi-continuous culture of the photobioreactor and harvesting of the gambierdiscus are carried out, specifically, when the gambierdiscus in the photobioreactor reaches the highest level of production capacity, layers of cells are slightly scraped up and uniformly mixed, then a part of algae liquid is discharged through a drain valve, and the remaining part is taken as an algae species of the next batch of culture; and the drained algae liquid is poured into a seawater barrel, precipitating is carried out for a period of time, supernatant is discarded, and centrifuging is carried out at the bottom to obtain wet and heavy algae.

Description

technical field [0001] The invention relates to the technical field of cultivation and production of algae gambiae, in particular to a large-scale continuous culture method for algae gambiae. Background technique [0002] As an important link in the food chain, microalgae is not only a biomass resource with potential for development to provide convenience for human beings, but also may produce biotoxins through the food chain biomagnification, and ultimately endanger human health. For example, paralytic shellfish poison produced by Alexandrium sp., okadaic acid produced by Prorocentrum lima, and sigatoxin and spinotoxin produced by gambiae. These toxins produced by microalgae have seriously endangered the life and health of fishermen and coastal residents who feed on seafood, and hindered the development of fishery economy. However, due to the limitations of research methods and technical means, the current research in this field is still in its infancy, and the biological ...

AI Technical Summary

Problems solved by technology

Due to the attached growth and long growth cycle of Gambiae algae, it is difficult to purify and cultivate in a large scale, and the traditional large-scale microalgae process cannot be fully applied. Therefore, a suitable large-scale cultivation process for Gambiae is needed to fill this gap.

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