Large-scale continuous culture method of gambierdiscus

A cultivation method and expanded cultivation technology, applied in the field of large-scale continuous cultivation of algae gambiae, can solve the problems of incomplete application, difficult purification, large-scale cultivation, long growth cycle, etc., achieve weight reduction, increase density and vitality, The effect of easy operation

Pending Publication Date: 2021-12-21
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the attached growth and long growth cycle of Gambiae algae, it is difficult to purify and cultivate in a large scale, and the traditional la

Method used

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  • Large-scale continuous culture method of gambierdiscus
  • Large-scale continuous culture method of gambierdiscus
  • Large-scale continuous culture method of gambierdiscus

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Embodiment Construction

[0034] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer and clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0035] The selected raw materials of the present invention can be obtained through commercial channels. Such as figure 1 Shown, the cultivation method of gambiae in the present embodiment, at first is to the algae species expansion cultivation of gambiae in order to preserve the good alga seed source of sufficient state, then takes the algae liquid in the culture bottle and transfers to the photobioreactor to adjust to Cultivate and collect after suitable temperature and light.

[0036] Specifically include the following steps:

[0037] 1. Laboratory expansion of algae gambiae

[0038] 1. Configure liquid culture medium

[0039] Configure the required nutrient salts, using L1 medium (without adding ...

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Abstract

The invention relates to a large-scale continuous culture method of gambierdiscus. The large-scale continuous culture method comprises the following steps that 1) enlarged culture of an gambierdiscus algae species is carried out, specifically, the algae species is transferred to a slant cell culture flask, the algae species is horizontally placed for culture until an exponential phase, then transferring is carried out, and the procedure is repeated to keep the algae species at the highest productivity level; 2) photobioreactor culture of the gambierdiscus, specifically, exponential-phase algae liquid is poured into a barrel containing a liquid culture medium, the algae liquid is uniformly mixed in the barrel, and finally the algae liquid in the barrel is transferred into a photobioreactor for culture; the photobioreactor adopts a vertical drawer type structure; and 3) semi-continuous culture of the photobioreactor and harvesting of the gambierdiscus are carried out, specifically, when the gambierdiscus in the photobioreactor reaches the highest level of production capacity, layers of cells are slightly scraped up and uniformly mixed, then a part of algae liquid is discharged through a drain valve, and the remaining part is taken as an algae species of the next batch of culture; and the drained algae liquid is poured into a seawater barrel, precipitating is carried out for a period of time, supernatant is discarded, and centrifuging is carried out at the bottom to obtain wet and heavy algae.

Description

technical field [0001] The invention relates to the technical field of cultivation and production of algae gambiae, in particular to a large-scale continuous culture method for algae gambiae. Background technique [0002] As an important link in the food chain, microalgae is not only a biomass resource with potential for development to provide convenience for human beings, but also may produce biotoxins through the food chain biomagnification, and ultimately endanger human health. For example, paralytic shellfish poison produced by Alexandrium sp., okadaic acid produced by Prorocentrum lima, and sigatoxin and spinotoxin produced by gambiae. These toxins produced by microalgae have seriously endangered the life and health of fishermen and coastal residents who feed on seafood, and hindered the development of fishery economy. However, due to the limitations of research methods and technical means, the current research in this field is still in its infancy, and the biological ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/00C12R1/89
CPCC12N1/12C12M21/02C12M23/04C12M23/22
Inventor 王大志柳原
Owner XIAMEN UNIV
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