Method for preparing low-arsenic oyster protein peptide through targeted nano silica gel material adsorption

A technology of oyster protein peptide and nano-silica gel, which is applied in the field of oyster protein peptide, can solve the problems of limited arsenic removal rate and unsuitable arsenic removal, and achieves excellent removal effect, excellent effect and low cost.

Pending Publication Date: 2021-12-31
无锡定象生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The doctoral dissertation "Research on the Distribution and Removal of Arsenic in Seaweed" disclosed the methods of removing arsenic, such as soaking in acid solution, soaking in alkali solution, soaking in boiling water, etc. The above methods have a certain effect on the removal of arsenic in seaweed. However, it is not suitable for the removal of arsenic in powdered protein peptides; and the above-mentioned method has a limited removal rate for arsenic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 46

[0114] Under a nitrogen atmosphere, a solution of 3-mercaptopropyltrimethoxysilane (0.16 mol) and allyl chloride (0.32 mol) was stirred under reflux for 2 h, during which 0.05 g of AIBN was added every 15 min. Excess allyl chloride was removed under reduced pressure, then a solution of 2-mercapto 1-sodium sulfide ethane (0.16mol) in methanol (50ml) was added, the resulting solution was refluxed for 2h and sodium methoxide (0.16mol) was added. After 15 min, 1-chloro, 3-thioacetylpropane (0.16 mol) was added, and the mixture was heated to reflux for 2 h. Morpholine (0.16 mol) was added and the mixture was refluxed for 2h. The sodium chloride was filtered off and the resulting solution was added to a stirred mixture of silica gel (120 g, 150-300 μm, 100 OA) and xylene (320 ml). The resulting mixture was heated with stirring at 120 °C for 1 h, during which time methanol was distilled off by a Dean-Stark apparatus. Add 3-mercaptopropyltrimethoxysilane (0.05mol), and continue heat...

Embodiment 1B

[0117] Taking oyster protein peptide powder from a certain company in Hubei as a sample, after testing, it contained 12.78 mg / kg of arsenic. Take 30 g of the oyster protein peptide powder, dissolve it with 20 wt % phosphoric acid solution to 100 ml, add 5 g of the Adsorbent, reflux and stir at 80°C for 2 hours, and filter after cooling to room temperature. The filter residue is the adsorbent that has adsorbed arsenic, and the filtrate is oyster protein peptide powder solution. Put the filtrate in a vacuum drying oven to dry for 24 hours. Refer to GB5009 for sampling. 11-2014 The second method of the first article was used to measure the total arsenic. After the adsorption, the arsenic content in the oyster peptide powder was reduced to 0.89mg / kg.

[0118] Note: The concentration of the acid solution is the mass concentration, the same below.

Embodiment 1C

[0120] Using the targeted nano-silica gel material in Example 1A as an adsorbent, it is applied to the removal of arsenic in oyster protein peptide powder;

[0121] Taking oyster protein peptide powder from a company in Shandong Province as a sample, it was detected that it contained 7.94 mg / kg of arsenic. Take 10 g of the oyster protein peptide powder, dissolve it with 35% hydrochloric acid solution to 100 ml, and add 5 g of it to the above-mentioned Example 1A reflux and stir at 60°C for 4 hours, and filter after cooling to room temperature. The filter residue is the adsorbent that has adsorbed arsenic, and the filtrate is oyster protein peptide powder solution. Put the filtrate into a freeze dryer to dry for 48 hours. Refer to GB5009 for sampling. 11-2014 The second method of the first article was used to measure the total arsenic. After the adsorption, the arsenic content in the oyster peptide powder was reduced to 0.66mg / kg.

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Abstract

The invention belongs to the technical field of food processing, and particularly relates to application of a targeted nano silica gel material to removal of heavy metal arsenic in oyster protein peptide powder, and a preparation method of the oyster protein peptide powder with low arsenic content by adopting targeted nano silica gel material. The preparation method comprises the following steps: adding a targeted nano silica gel material into an aqueous solution or an acid solution containing oyster protein crude peptide, refluxing and stirring at 0-100 DEG C for 0.25-12 hours, and filtering; and drying the filtering solution, obtaining the oyster protein peptide with the arsenic content smaller than 1 ppm by adding the filtrate into the oyster protein peptide powder, and converting the oyster protein peptide powder originally containing 12 mg / kg of arsenic into a product containing 1 mg / kg or below of arsenic, so that the content of arsenic in the oyster protein peptide powder is greatly reduced, and the food safety is remarkably improved. The method is simple and convenient to operate, the used reagent is easy to obtain, the adsorption cost is low, the effect is excellent, the problem about arsenic removal in the oyster protein peptide powder is solved, and the excellent arsenic removal effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of food processing, and specifically relates to the application of a specific targeted nano-silica gel material to the removal of arsenic in oyster protein peptides, and also relates to a method for using the targeted nano-silica gel material to remove arsenic in oyster protein peptides. A method for preparing oyster protein peptides with low arsenic content and oyster protein peptides obtained by the method. Background technique [0002] The main surface of the harm of arsenic to the human body is: inhibiting the function of enzymes in the body, interfering with the production of ATP; teratogenic and carcinogenic; people should try to reduce the intake of arsenic in the process of eating. The food processing industry also needs to reduce the content of arsenic in food as much as possible to make it meet the standard, so as to ensure food safety and human diet health. [0003] As for the removal methods of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20C12P21/06C07K1/22
CPCA23L5/273C11B3/001C11B3/006C11B3/10C11B3/12C11B3/008A23J3/341A23V2002/00A23V2300/02A23V2250/55
Inventor 杨南超
Owner 无锡定象生物科技有限公司
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