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Method for purifying bacterial cellulose membrane and application thereof

A bacterial cellulose membrane and pure water technology, which is applied in the field of bacterial cellulose membrane purification, can solve the problem of bacterial cellulose total bacterial count, appearance permeability, cytotoxicity not meeting the requirements, bacterial cellulose membrane damage, yellowing and discoloration, etc. problem, achieve the effect of avoiding discoloration, avoiding oxidation, and high concentration

Pending Publication Date: 2022-01-04
SHAN DONG NAMEIDE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the existing bacterial cellulose in the prior art, food coloring, preservatives, flavors and other ingredients are added, the total bacterial count, appearance permeability and cytotoxicity in the bacterial cellulose do not meet the requirements, and yellowing, discoloration and wrinkling are avoided. Therefore, the present invention proposes a method for purifying bacterial cellulose membrane and its application. The experiment found for the first time that the combination of hot alkali and normal temperature alkali washing can remove more cellulose without destroying the structure of the bacterial cellulose membrane. impurities, and at the same time avoid damage to the bacterial cellulose membrane during the subsequent hydrogen peroxide cleaning process

Method used

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  • Method for purifying bacterial cellulose membrane and application thereof
  • Method for purifying bacterial cellulose membrane and application thereof
  • Method for purifying bacterial cellulose membrane and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) The fermented membrane is washed with pure water in the purification tank, and the air volume of the purification tank is set at 8m 3 / min, rinse for 45min.

[0043](2) Add hot NaOH solution to the film after the above treatment, the temperature is 65°C, the concentration is 5‰, soak for 20min, and then send it into the blast for 5h, and the blast volume is 10m 3 / min.

[0044] (3) Replace with new NaOH solution at room temperature, temperature 25°C, concentration 2‰, soak for 40 minutes, then pour 1.5‰ hydrogen peroxide into the room temperature lye soaked with diaphragm, seal and soak for 2 hours.

[0045] (4) The membrane treated in the above steps was continued to be washed with pure water for 6 times, and the pH value inside and outside the membrane reached 6.9.

[0046] (5) Put the treated membrane into steam for 30 minutes.

[0047] Mouse fibroblasts were inoculated in a 96-well plate and cultured for 24 hours until the cells grew to confluence. Using MEM ...

Embodiment 2

[0050] (1) The fermented membrane is washed with pure water in the purification tank, and the air volume of the purification tank is set at 8m 3 / min, rinse for 45min.

[0051] (2) Add hot NaOH solution to the film after the above treatment, the temperature is 60°C, the concentration is 4‰, soak for 20min, and then send it into the blast for 5h, and the blast volume is 10m 3 / min.

[0052] (3) Replace with new NaOH solution at room temperature, temperature 10°C, concentration 1‰, soak for 60 minutes, then pour 1.5‰ hydrogen peroxide into the room temperature lye soaked with diaphragm, seal and soak for 2 hours.

[0053] (4) The membrane treated in the above steps was continued to be washed with pure water for 6 times, and the pH value inside and outside the membrane reached 6.9.

[0054] (5) Put the treated membrane into steam for 30 minutes.

[0055] The experiment found that the prepared bacterial cellulose membrane was transparent and colorless.

Embodiment 3

[0057] (1) The fermented membrane is washed with pure water in the purification tank, and the air volume of the purification tank is set at 8m 3 / min, rinse for 45min.

[0058] (2) Add hot NaOH solution to the film after the above treatment, the temperature is 70 ° C, the concentration is 10‰, soak for 20 minutes, and then send it into the blast for 5 hours, and the blast volume is 10m 3 / min.

[0059] (3) Replace with new NaOH solution at room temperature, temperature 30°C, concentration 3‰, soak for 30 minutes, then pour 1.5‰ hydrogen peroxide into the room temperature lye soaked with diaphragm, seal and soak for 2 hours.

[0060] (4) The membrane treated in the above steps was continued to be washed with pure water for 6 times, and the pH value inside and outside the membrane reached 6.9.

[0061] (5) Put the treated membrane into steam for 30 minutes.

[0062] The experiment found that the prepared bacterial cellulose film was flat, without air bubbles and colorless.

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Abstract

The invention relates to the field of biomedical material manufacturing, in particular to a method for purifying a bacterial cellulose membrane and application thereof. In order to solve the problems that edible pigments, preservatives, essences and other components are added into bacterial cellulose in the prior art, the total bacterial count, appearance permeability and cytotoxicity in the bacterial cellulose do not meet the requirements, and yellowing, discoloration, wrinkling, blistering and the like are avoided, the invention provides the method for purifying the bacterial cellulose membrane and application thereof. Experiments find for the first time that through cooperation of hot alkali and normal-temperature alkali washing, more impurities can be removed on the premise that the structure of the bacterial cellulose membrane is not damaged, and meanwhile damage to the bacterial cellulose membrane in the follow-up hydrogen peroxide cleaning process can be avoided. The mammalian fibroblasts are treated by the leach liquor of the membrane for biological reaction, and the result shows that the cell activity is greater than 90%.

Description

technical field [0001] The invention relates to the field of biomedical material manufacture, in particular to a method for purifying bacterial cellulose membrane and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The synthesis of bacterial cellulose is a multi-step reaction process precisely regulated by a large number of multi-enzyme complex systems. First, the synthesis of cellulose precursor uridine diphosphate glucose (UDPGlu), and then the oligomeric cellulose synthase complex continuously Glucopyranose residues are transferred from UDPGlu to newly generated polysaccharide chains to form α-(1→4)-D-glucan chains, which are secreted to the outside of...

Claims

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Application Information

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IPC IPC(8): C08J7/00C08L1/02A61K47/38A61L27/20A61L27/60A61L27/50A61L15/28
CPCC08J7/00A61K47/38A61L27/20A61L27/60A61L27/507A61L15/28C08J2301/02A61L2430/06A61L2430/34C08L1/02
Inventor 苏红霞张杰蔡玉文张秦刘景君
Owner SHAN DONG NAMEIDE BIOTECHNOLOGY CO LTD
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