Method for purifying bacterial cellulose membrane and application thereof
A bacterial cellulose membrane and pure water technology, which is applied in the field of bacterial cellulose membrane purification, can solve the problem of bacterial cellulose total bacterial count, appearance permeability, cytotoxicity not meeting the requirements, bacterial cellulose membrane damage, yellowing and discoloration, etc. problem, achieve the effect of avoiding discoloration, avoiding oxidation, and high concentration
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[0041] Example 1:
[0042] (1) The fermented membrane is washed with pure water in the purification barrel, and the flushing air volume of the purification barrel is set at 8m 3 / min, rinse for 45min.
[0043](2) Add hot NaOH solution to the film after the above treatment, the temperature is 65℃, the concentration is 5‰, soaked for 20min, and then sent to blast for 5h, and the blast volume is 10m 3 / min.
[0044] (3) Replace with a new normal temperature NaOH solution, the temperature is 25 ℃, the concentration is 2‰, soak for 40min, then pour 1.5‰ hydrogen peroxide into the normal temperature lye soaked with the diaphragm, seal and soak for 2h.
[0045] (4) The membrane treated by the above steps was continuously washed with pure water for 6 times, and the pH value inside and outside the membrane reached 6.9.
[0046] (5) Pass the treated membrane into steam for 30min.
[0047] The mouse fibroblasts were inoculated in a 96-well plate and cultured for 24 hours until the ce...
Example Embodiment
[0049] Example 2
[0050] (1) The fermented membrane is washed with pure water in the purification barrel, and the flushing air volume of the purification barrel is set at 8m 3 / min, rinse for 45min.
[0051] (2) Add hot NaOH solution to the film after the above treatment, the temperature is 60°C, the concentration is 4‰, soaked for 20min, and then sent to blast for 5h, and the blast volume is 10m 3 / min.
[0052] (3) Replace with a new normal temperature NaOH solution, the temperature is 10 ℃, the concentration is 1‰, soak for 60min, then pour 1.5‰ hydrogen peroxide into the normal temperature lye soaked with the diaphragm, seal and soak for 2h.
[0053] (4) The membrane treated by the above steps was continuously washed with pure water for 6 times, and the pH value inside and outside the membrane reached 6.9.
[0054] (5) Pass the treated membrane into steam for 30min.
[0055] The experiment found that the prepared bacterial cellulose membrane was transparent and colorl...
Example Embodiment
[0056] Example 3
[0057] (1) The fermented membrane is washed with pure water in the purification barrel, and the flushing air volume of the purification barrel is set at 8m 3 / min, rinse for 45min.
[0058] (2) Add hot NaOH solution to the film after the above treatment, the temperature is 70°C, the concentration is 10‰, soaked for 20min, and then sent to blast for 5h, and the blast volume is 10m 3 / min.
[0059] (3) Replace with a new normal temperature NaOH solution, the temperature is 30 ℃, the concentration is 3‰, soak for 30min, then pour 1.5‰ hydrogen peroxide into the normal temperature lye soaked with the diaphragm, seal and soak for 2h.
[0060] (4) The membrane treated by the above steps was continuously washed with pure water for 6 times, and the pH value inside and outside the membrane reached 6.9.
[0061] (5) Pass the treated membrane into steam for 30min.
[0062] Experiments found that the prepared bacterial cellulose membrane had smooth appearance, no ai...
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