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Application of pectolinarin in preparation of neuroinflammation inhibitors

A technology of neuroinflammation and sagoside, applied in the field of drug research

Pending Publication Date: 2022-01-14
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current research shows that thistle has a series of biological activities such as antibacterial, hemostasis, lowering blood pressure, and antitumor. It is mainly used to treat various bleeding, inflammation, and hypertension in clinical practice. , anti-oxidation, anti-tumor and other functions have been confirmed, but whether it can inhibit neuroinflammation is unknown

Method used

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  • Application of pectolinarin in preparation of neuroinflammation inhibitors
  • Application of pectolinarin in preparation of neuroinflammation inhibitors
  • Application of pectolinarin in preparation of neuroinflammation inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Sigmarin on microglial BV 2 effect on cell viability

[0028] In order to study whether signarin has an inhibitory effect on neuroinflammation, firstly, whether sardinin affects BV 2 The biological activity of cells was tested. The specific method is as follows:

[0029] will BV 2 The cells were spread in a 96-well plate, and when the density grew to 70%-80%, different concentrations of sardinin 0 μM, 0.1 μM, 1 μM, 10 μM, 50 μM, and 100 μM were added to continue the culture, and the MTT method was used to detect the cells after 48 hours Sigmarin on BV 2 effect on cell viability. All data were analyzed using the method of mean ± standard deviation, and each analysis was repeated at least three times. The result is as figure 1 shown.

[0030] figure 1 The test results showed that 0-100μM sigalin had an effect on BV 2 Cell growth and viability were not significantly affected, and follow-up experiments can be carried out.

Embodiment 2

[0032] Effect of sardin on LPS-induced microglial BV 2 Effects on mRNA expression levels of inflammatory factors TNF-α and IL-6 released by activation

[0033] Firstly, a model of neuroinflammation was established. Lipopolysaccharide (LPS) is an endotoxin that can bind to Toll-like receptor 4 (TLR4) on the surface of microglia to generate a cascade reaction and induce neuroinflammation. Use this as a model to detect related inflammatory factors. The specific operation process is as follows:

[0034] to BV 2 The cells were pretreated with different concentrations of sardin (50 μM, 100 μM) for 1 h, then treated with 1 μg / ml LPS for 24 h, and DNA was extracted for qPCR detection. All data were analyzed using the method of mean ± standard deviation, and each analysis was repeated at least three times. The result is as figure 2 shown.

[0035] Depend on figure 2 It can be seen that compared with the blank group, the expression of inflammatory factors in the LPS group incre...

Embodiment 3

[0037] Effect of sardin on LPS-induced microglial BV 2 Effects on the protein expression levels of the released inflammatory factors TNF-α and IL-6 after activation

[0038] to BV 2 The cells were pretreated with different concentrations of silymarin (50 μM, 100 μM) for 1 h, and then treated with 1 μg / ml LPS for 24 h, and the supernatant was taken to detect the expression of tumor necrosis factor TNF-α and IL-6 at the protein level using ELISA kits. relative expression. All data were analyzed using the method of mean ± standard deviation, and each analysis was repeated at least three times. The result is as image 3 shown.

[0039] Depend on image 3 It can be seen that compared with the blank group, the protein expression levels of TNF-α and IL-6 in the LPS stimulation group were significantly increased; after the addition of sternin, the protein expression levels of TNF-α and IL-6 in the drug-dosed group were significantly reduced .

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Abstract

The invention belongs to the technical field of pharmaceutical researches, and particularly provides application of pectolinarin in preparation of neuroinflammation inhibitors. Cell experiments prove that the pectolinarin can inhibit mRNA expression and protein expression of inflammatory factors TNF-alpha and IL-6 activated and released by LPS-induced microglial cells BV2 and oxidative stress reaction generated by activation of the microglial cells BV2.

Description

technical field [0001] The invention belongs to the technical field of drug research, and specifically discloses the use of sardin in the preparation of neuroinflammation inhibitors. Background technique [0002] The immune system is very important in the regulation of inflammation. It is the body's first line of defense against all external stimuli and invasions. It also has the function of resisting pathogens and repairing damaged tissues, but chronic induction may lead to damage to the body. Neuroinflammation, that is, inflammation that occurs in the central nervous system, is considered to be a pathological feature of many diseases, such as Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, etc. Many evidences have shown that neuroinflammation plays a key role in the process of neuron degeneration, and the pathological features of neurodegenerative diseases have verified this view. [0003] Most neurodegenerative diseases have a common feature, tha...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K36/28A61P25/00A61P29/00A61P25/16A61P25/28A61P21/00
CPCA61K31/7048A61K36/28A61P25/00A61P29/00A61P25/16A61P25/28A61P21/00
Inventor 程彬峰杨海杰王磊丰肖简少钦
Owner XINXIANG MEDICAL UNIV
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