Method for detecting genotyping of 152 gene loci related to child allergy
A gene locus and detection method technology, applied in the field of genotyping detection of 152 gene loci, can solve problems affecting children's life and health, achieve early prediction and prevention of allergic diseases, high comparison rate, and construction cycle short effect
Pending Publication Date: 2022-01-14
飞科易特广州基因科技有限公司
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Problems solved by technology
However, these tests are usually performed after the patient has shown a severe allergic reaction, which seriously affects the life and health of the child
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Embodiment
[0058] This embodiment is a detection method for genotyping of 152 gene loci related to children's allergy. The detection method includes the following steps:
[0059] (a) Collect the saliva of the test subject and extract the DNA:
[0060] Collect the saliva of the test object, add the extraction buffer solution to the saliva sample, and repeatedly blow and mix the extraction buffer solution, centrifuge at 8000×g for 5 minutes, discard the supernatant, and repeat this step; add the lysate and proteinase K to the obtained precipitate , thoroughly suspend the precipitate and mix it well, place it at room temperature for 30 minutes, and invert the centrifuge tube several times during this period; add 10 uL of an aqueous solution with a concentration of 10 mg / mL RNase to the obtained mixture, and let it stand at 37°C for 10 minutes; Add an equal volume of phenol-chloroform mixed solution to the supernatant, the volume ratio of phenol and chloroform is 1.0, mix thoroughly, and cen...
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The invention discloses a method for detecting genotyping of 152 gene loci related to child allergy. The method comprises the following steps of: collecting saliva or an oral swab of a detected object, and extracting DNA (Deoxyribonucleic Acid); performing PCR (Polymerase Chain Reaction) amplification on 152 gene loci by taking the obtained DNA as a template, and performing magnetic bead purification on an amplified product; performing index sequence connection on the purified product, and performing magnetic bead purification again to obtain a PCR library; and performing gene sequencing on the PCR library, and obtaining genotyping results of 152 gene loci related to children allergy according to a sequencing result. 152 gene loci in the detection method can comprehensively evaluate the allergy heredity condition of children from the biological perspective and help parents to know the allergy risk of the children; the guide is scientific, and serious allergy of the children is effectively reduced; early discovery, early intervention and early treatment are achieved on allergens of the children; and in addition, the detection method is simple to operate, short in time and high in efficiency.
Description
technical field [0001] The invention relates to the technical field of genotyping technology, in particular to a detection method for genotyping of 152 gene loci related to children's allergy. Background technique [0002] An allergen is a foreign protein or hapten that induces the production of specific IgE antibodies and triggers an allergic response. Most allergenic proteins are recognized by sensitive individuals and trigger an allergic reaction. Not all proteins in allergens are allergenic, which is related to individual genetic susceptibility and the complexity of allergens. The interaction between allergens and IgE is the basis for the pathogenesis of allergic diseases. Most allergens are composed of proteins, and these protein-like allergens have the same property as common antigens, namely stimulating the body to produce immunoglobulins. At the same time, these allergens, because of their special biochemical and physical and chemical characteristics, cause sensiti...
Claims
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Login to View More IPC IPC(8): C12Q1/6883C12Q1/686C12N15/11C40B40/06C40B50/06
CPCC12Q1/6883C12Q1/686C40B40/06C40B50/06C12Q2563/143C12Q2563/149
Inventor 钟玙沄谭鸣林德玲杜英文强
Owner 飞科易特广州基因科技有限公司