Fluorescence and resonance Rayleigh scattering light dual-element signal probe for detecting tetracycline, and preparation method and application thereof

A technology of resonant Rayleigh scattering and signal probes, which is applied in the directions of fluorescence/phosphorescence, chemical instruments and methods, and measurement of scattering characteristics. The process is simple and the effect of controlling food quality and safety

Pending Publication Date: 2022-01-14
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these detection methods are costly and complicated to operate, and it is difficult to meet the requirements of rapid detection.

Method used

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  • Fluorescence and resonance Rayleigh scattering light dual-element signal probe for detecting tetracycline, and preparation method and application thereof
  • Fluorescence and resonance Rayleigh scattering light dual-element signal probe for detecting tetracycline, and preparation method and application thereof
  • Fluorescence and resonance Rayleigh scattering light dual-element signal probe for detecting tetracycline, and preparation method and application thereof

Examples

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preparation example Construction

[0031] This application provides a method for preparing a fluorescence and resonance Rayleigh scattered light binary signal probe for detecting tetracycline, including:

[0032] The raw materials including citric acid, ethylenediamine and water are first mixed, heated for the first reaction, and then the first separation is carried out to obtain the carbon dot solution;

[0033] The carbon dot solution, the guanosine monophosphate solution, the cerium nitrate hexahydrate solution and the buffer solution are mixed for the second time, and after the second reaction, the fluorescence and resonance Rayleigh scattered light for detecting tetracycline are obtained through the second separation. Meta signal probe.

[0034] In a preferred embodiment, the first mixing includes: mixing the citric acid, the ethylenediamine and the water until the citric acid is dissolved.

[0035] In a preferred embodiment, the temperature of the first reaction is 150-200°C, and the time is 4-6h.

[00...

Embodiment 1

[0048] This embodiment provides a method for preparing a fluorescent and resonant Rayleigh scattering binary signal probe for detecting tetracycline, which includes the following steps:

[0049] (1) Preparation of carbon dots: Weigh 1.2670 g of citric acid into a beaker, add 1608 μL of ethylenediamine and 30 mL of water to completely dissolve the citric acid. It was then transferred to an autoclave and kept at 200 °C for 5 h. After it was taken out and cooled to room temperature, it was dialyzed with a dialysis bag (1000Da) for 24 hours to obtain a carbon dot solution (0.064mg / mL), and stored at 4°C.

[0050] (2) Preparation of carbon dots@guanosine monophosphate / cerium (CDs@GMP / Ce) probe: Prepare cerium nitrate hexahydrate solution (25mmol / L) and guanosine monophosphate solution (20mmol / L) respectively 10mL, then mix the guanosine monophosphate solution and the carbon dots described in (1) with a magnetic stirrer, then add the prepared cerium nitrate hexahydrate solution int...

Embodiment 2

[0052] This example provides a method for preparing a fluorescent and resonant Rayleigh scattering binary signal probe for the detection of tetracycline. The difference from Example 1 is that the carbon dots are prepared under the condition of 150°C for 6 hours, and the carbon dots The @guanosine monophosphate / cerium (CDs@GMP / Ce) probe was prepared by centrifugation at 8000rpm / min for 10min. It can be understood that the preparation of carbon dots can also be under other temperature and time conditions, and the preparation of carbon dots@guanosine monophosphate / cerium (CDs@GMP / Ce) probes can also be under other centrifugal speed and time conditions.

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Abstract

The invention provides a fluorescence and resonance Rayleigh scattering light dual-element signal probe for detecting tetracycline, and a preparation method and application thereof. The preparation method of the fluorescence and resonance Rayleigh scattering light dual-element signal probe for detecting tetracycline comprises the following steps: carrying out first mixing on raw materials including citric acid, ethylenediamine and water, heating to carry out first reaction, and then carrying out first separation to obtain a carbon dot solution; and carrying out second mixing on the carbon dot solution, a guanosine monophosphate solution, a cerium nitrate hexahydrate solution and a buffer solution, carrying out second reaction, and carrying out second separation to obtain the probe. The fluorescence and resonance Rayleigh scattering light dual-element signal probe for detecting tetracycline is prepared by using the preparation method. The application of the probe is used for detecting tetracycline. The probe provided by the invention has dual signal response of fluorescence and resonance Rayleigh scattering light to tetracycline, and the dual signal can be detected by a single instrument fluorescence spectrometer, so that rapid dual signal cross validation can be realized when the probe is used for tetracycline detection.

Description

technical field [0001] The present application relates to the field of food safety analysis, in particular to a fluorescence and resonance Rayleigh scattered light binary signal probe for detecting tetracycline and its preparation method and application. Background technique [0002] Tetracyclines are a class of broad-spectrum antibiotics, which belong to the derivatives of polycyclic tetracenecarboxamide core, and have good antibacterial activity against pathogenic microorganisms. Tetracyclines are widely used in animal husbandry because of their low cost, excellent antibacterial properties, and good therapeutic effects. However, tetracyclines are difficult to degrade and may accumulate in soil, water, foods such as meat, eggs and milk. Excessive tetracycline use can lead to tetracycline resistance in livestock and humans, which has a great adverse effect on ecosystems and human health. Studies have found that regular intake of tetracycline can cause liver and kidney dama...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/51C09K11/65B82Y20/00B82Y40/00
CPCG01N21/643G01N21/51C09K11/65B82Y20/00B82Y40/00
Inventor 刘石刚杨淑娟邓洁红石星波
Owner HUNAN AGRICULTURAL UNIV
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