SNP marker site related to adaptability of horse plateau hypoxia and application of SNP marker site
A technique for marking sites and plateau hypoxia, which is applied in the field of genetic engineering to achieve the effect of reducing the concentration
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[0022] 1. Collection of experimental animals and blood samples
[0023] The present invention is used for whole genome resequencing samples, respectively from Qusong County, Tibet Autonomous Region (4500m, 20 horses), Diqing Prefecture, Yunnan Province (3500m, 10 horses), Zuogong County, Tibet Autonomous Region (3500m, 10 horses), Yunnan Province Laojun Mountain (2500m, 10 horses), Lijiang City, Yunnan Province (2500m, 10 horses), Yimen County, Yunnan Province (1500m, 10 horses), Zhaotong City, Yunnan Province (1500m, 10 horses), Pingbian County, Yunnan Province (500m , 10 horses), Yingjiang County, Yunnan Province (500m, 10 horses).
[0024] A total of 174 Diqing Tibetan horse samples were used for the analysis of the association between genotype and blood physiology, and the samples came from Diqing Prefecture, Yunnan.
[0025] 5ml of blood was collected from the jugular vein of horses using vacuum blood collection tubes containing EDTA anticoagulant, for a total of 274 sam...
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