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Method for removing endotoxin in bacterial cellulose

A bacterial cellulose and endotoxin technology, which is applied in the post-treatment of cellulose pulp, fiber raw material treatment, pulp fat/resin/pitch/wax removal, etc., can solve the problems of poor removal effect and speed up the purification process Effect

Active Publication Date: 2022-01-21
SHAN DONG NAMEIDE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used methods for removing endotoxin include activated carbon adsorption, extraction, ultrafiltration, ion exchange chromatography, etc., but the removal effect is not good.

Method used

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  • Method for removing endotoxin in bacterial cellulose
  • Method for removing endotoxin in bacterial cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Soak the sliced ​​bacterial cellulose in 2% sodium hydroxide solution by mass fraction, replace the sodium hydroxide solution four times and soak for four hours each time; kill the bacteria so that the endotoxin is completely released and also accelerate the bacterial cellulose the process of purification;

[0035] (2) soak bacterial cellulose in 10% ethanol by volume fraction, and utilize 10% ethanol to dissolve the endotoxin in the porous structure;

[0036] (3) Add mass fraction 2% sodium hydroxide solution and positively charged polysulfone resin polymer to the bacterial cellulose processed in the second step, the polysulfone resin polymer has good hydrophilicity and can effectively adsorb and filter In addition to endotoxin in liquid and bacterial cellulose, and 2% sodium hydroxide solution will completely kill the endotoxin adsorbed;

[0037] (4) Soak the treated bacterial cellulose with water for injection to make it reach a pH range of 7±1.

[0038] (5) Sa...

Embodiment 2

[0040] (1) Soak the pieced bacterial cellulose in 3% sodium hydroxide solution by mass fraction, replace the sodium hydroxide solution four times and soak for four hours each time; kill the bacteria so that the endotoxin is completely released and also accelerate the bacterial cellulose the process of purification;

[0041] (2) soak bacterial cellulose in 15% ethanol by volume fraction, and utilize 15% ethanol to dissolve the endotoxin in the porous structure;

[0042] (3) The bacterial cellulose processed in the second step is added to a mass fraction of 3% sodium hydroxide solution and a positively charged polysulfone resin polymer. The polysulfone resin polymer has good hydrophilicity and can effectively adsorb and filter In addition to endotoxin in liquid and bacterial cellulose, and 3% sodium hydroxide solution will completely kill the endotoxin adsorbed;

[0043] (4) Soak the treated bacterial cellulose with water for injection to make it reach a pH range of 7 ± 1;

[00...

Embodiment 3

[0046] (1) Soak the sliced ​​bacterial cellulose in 1% sodium hydroxide solution by mass fraction, replace the sodium hydroxide solution four times, soak for four hours each time, kill the bacteria so that the endotoxin is completely released and also accelerate the bacterial fiber The process of element purification;

[0047] (2) Soak the bacterial cellulose in 5% ethanol by volume fraction, and use 5% ethanol to dissolve the endotoxin in the porous structure;

[0048] (3) Add mass fraction 1% sodium hydroxide solution and positively charged polysulfone resin polymer to the bacterial cellulose processed in step (2), the polysulfone resin polymer has good hydrophilicity and can effectively adsorb Filter out endotoxins in liquid and bacterial cellulose, and 1% sodium hydroxide solution will completely kill the adsorbed endotoxins;

[0049] (4) Soak the treated bacterial cellulose with water for injection to make it reach a pH range of 7±1;

[0050] (5) Samples were randomly s...

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Abstract

The invention relates to the technical field of medical instruments, in particular to a method for removing endotoxin in bacterial cellulose. The method comprises the following steps: (1) soaking sliced bacterial cellulose in a sodium hydroxide solution, and replacing the sodium hydroxide solution for soaking for multiple times; (2) soaking bacterial cellulose in an ethanol solution, and dissolving endotoxin in the porous structure; (3) adding a sodium hydroxide solution and a polysulfone resin polymer with positive charges into the bacterial cellulose treated in the step (2); and (4) soaking the treated bacterial cellulose in water for injection until the pH value is 7 + / -1. According to the method, the endotoxin in the bacterial cellulose is removed, the purification process of the bacterial cellulose is accelerated, the mechanical property and physical and chemical aspects of the bacterial cellulose are not affected, and the endotoxin content of the obtained bacterial cellulose is far smaller than the specified content.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a method for removing endotoxin in bacterial cellulose. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] At present, the three types of medical device products are strictly controlled for endotoxins. Bacterial cellulose is of high value as a new type of natural nanomaterial. Bacterial cellulose has good air permeability and good biocompatibility and has broad application in the medical industry. prospect. However, the removal of endotoxin is still a very immature direction in the bacterial cellulose material industry. The bacterial cellulose has a porous structure that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21C9/00D21C5/00
CPCD21C9/08D21C9/083D21C9/086D21C5/00Y02A50/30
Inventor 张杰柴谦张秦苏红霞刘景君
Owner SHAN DONG NAMEIDE BIOTECHNOLOGY CO LTD
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