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Periosteum-bone complex for reconstruction of soft tissue-bone immune repair environment and preparation method of periosteum-bone complex

An immune repair and complex technology, applied in tissue regeneration, prosthesis, medical science, etc., can solve the problem of lack of periosteum-bone complex, and achieve coordination of early immune regulation of osteogenesis-angiogenesis events, good biological phase. Capacitance, the effect of promoting bone repair

Active Publication Date: 2022-01-28
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are no reports on the preparation of periosteum-bone complex

Method used

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  • Periosteum-bone complex for reconstruction of soft tissue-bone immune repair environment and preparation method of periosteum-bone complex
  • Periosteum-bone complex for reconstruction of soft tissue-bone immune repair environment and preparation method of periosteum-bone complex
  • Periosteum-bone complex for reconstruction of soft tissue-bone immune repair environment and preparation method of periosteum-bone complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: The periosteum-bone complex and its preparation method aimed at rebuilding soft tissue-bone immune repair environment

[0045] (1) Take materials

[0046] Take the femur of a healthy adult Large White pig within 12 hours of slaughter, separate the metaphysis, collect the femoral shaft part, remove the internal bone marrow cavity, and divide the femoral shaft into 10mm*8mm pieces;

[0047] (2) Pretreatment

[0048] Take a certain amount of flaky bone, place it on a low-temperature (4°C) shaker at 100 rpm, rinse with PBS buffer containing protease inhibitors for 3 times, 10 minutes each time, to remove blood, attached fat fragments and other impurities;

[0049] (3) Decalcification

[0050] The above pretreated flaky bone was placed in 20% (w / v) EDTA-2Na solution for ultrasonic decalcification for 12 days, the ultrasonic parameter was 40kHZ, and the temperature was 22°C;

[0051] (4) Acquisition of periosteum-bone complex

[0052] ① Three cycles of liqui...

example 1

[0063] 1. Gross view and decellularization evaluation of periosteum-bone complex scaffold in Example 1

[0064] The general appearance and CT scan of the periosteum-bone complex scaffold, such as figure 1 .

[0065] 2. Decellularization Evaluation of the Periosteum-bone Complex Scaffold in Example 1

[0066] H&E staining showed that there were no cells and no nuclear components remained, and DNA quantitative detection almost contained no DNA components, such as figure 2 .

[0067] 3. Microstructural evaluation of the periosteum-bone complex scaffold in Example 1

[0068] Scanning electron microscope observation collagen arrangement, bone crystal structure and periosteum-bone interface connection structure of the present invention, such as image 3 .

[0069] 4. Surface topography and mechanical evaluation of the periosteum-bone complex scaffold in Example 1

[0070] Atomic force microscope observes the microscopic surface topography of the present invention, such as F...

Embodiment 2

[0081] Embodiment 2: The periosteum-bone complex and its preparation method aimed at rebuilding soft tissue-bone immune repair environment

[0082] (1) Take materials

[0083] Take the femur of a healthy adult Large White pig within 12 hours of slaughter, separate the metaphysis, collect the femoral shaft part, remove the internal bone marrow cavity, and divide the femoral shaft into 10mm*8mm pieces;

[0084] (2) Pretreatment

[0085] Take a certain amount of flaky bone, place it on a low-temperature (4°C) shaker at 100 rpm, rinse with PBS buffer containing protease inhibitors for 3 times, 10 minutes each time, to remove blood, attached fat fragments and other impurities;

[0086] (3) Decalcification

[0087] The above pretreated flaky bone was placed in 20% (w / v) EDTA-2Na solution for ultrasonic decalcification for 12 days, the ultrasonic parameter was 40kHZ, and the temperature was 22°C;

[0088] (4) Acquisition of periosteum-bone complex

[0089] ① Three cycles of liqui...

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Abstract

The invention discloses a periosteum-bone complex for reconstructing a soft tissue-bone immune repair environment and a preparation method thereof. The preparation method comprises steps: cutting and proofing, repeatedly rinsing with deionized water, freezing and thawing with liquid nitrogen, performing ultrasonic decalcification, and treating with a PBS buffer solution containing a protease inhibitor, a PBS buffer solution containing Triton X-100, a PBS buffer solution containing SLES, a PBS buffer solution containing DNase I and a Tris-HCl buffer solution to obtain the cell-free periosteum-bone complex material. The complex retains a good periosteum-bone space structure and extracellular active components, provides a 'chondrometrium-sclerotin' biphasic interface, effectively blocks excessive inflammation infiltration of a soft tissue source, participates in early immune remodeling of soft tissue-bone injury, forms a benign immune-repair microenvironment by combining own osteogenesis and vascularization advantages of the complex. A new thought is provided for promoting bone tissue regeneration and repair by a biological material.

Description

technical field [0001] The present invention mainly relates to the biological field for bone tissue regeneration and repair, specifically a periosteum-bone complex and its preparation method aimed at rebuilding the soft tissue-bone immune repair environment. The impact of bone repair, rational use of the advantages of spatial composite mode scaffolds to promote bone regeneration and repair. Background technique [0002] Bone defects caused by trauma, tumors and infections are common clinical diseases that affect human health, especially multiple complex bone defects, which often require repeated treatment, causing heavy psychological pressure and economic burden on individuals, and affecting social stability. The potential impact is an urgent problem to be solved in clinical orthopedics. [0003] In recent years, the substitution therapy of biomaterials has gradually attracted people's attention. Among them, high-rigidity single-phase bone substitutes (such as bioceramics a...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/50A61L2430/02A61L2430/40A61L2430/34
Inventor 林贤丰赵晨晨范顺武
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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