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Method for high-sensitivity detection of thiamphenicol based on up-conversion nano material off-on type fluorescence sensor

A technology of thiamphenicol and fluorescent probe, which is applied in the field of fluorescent nanomaterials and fluorescent sensing, can solve the problems of unfavorable biological sample analysis and detection, strong background fluorescence, poor selectivity, etc., and achieve friendly detection, high sensitivity and accuracy high effect

Active Publication Date: 2022-02-01
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low sensitivity and poor selectivity of the existing thiamphenicol detection method, and some fluorescent materials excited by ultraviolet and visible wavelengths have strong background fluorescence in biological analysis, which is not conducive to the analysis and detection of biological samples, etc. question

Method used

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  • Method for high-sensitivity detection of thiamphenicol based on up-conversion nano material off-on type fluorescence sensor
  • Method for high-sensitivity detection of thiamphenicol based on up-conversion nano material off-on type fluorescence sensor
  • Method for high-sensitivity detection of thiamphenicol based on up-conversion nano material off-on type fluorescence sensor

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Embodiment 1

[0023] This embodiment is NaYF 4 :Yb, the preparation method of Er-gold nanoparticle fluorescent probe is specifically completed in the following steps:

[0024] 1. Preparation of oleate precursor: Add a mixed solution of 20mmol rare earth metal chloride, 60mmol sodium oleate, 30mL distilled water, 40mL ethanol and 70mL n-hexane into the flask, heat to 70°C for 4h under magnetic stirring, and then cool to At room temperature, pour the mixed solution into a separatory funnel and let it stand for 5 minutes, wash the upper layer liquid three times with distilled water, and dry it in a water bath at 80°C. The obtained solid waxy substance is an oleate precursor containing Y, Er and Yb elements ; The rare earth metal chloride described in step one is YCl 3 , ErCl 3 and YbCl 3 ;

[0025] 2. Preparation of NaYF 4 :Yb, Er: combine NaF, Y(OA) 3 , Yb(OA) 3 、Er(OA) 3 According to the material ratio of 1:0.785:0.20:0.015 mixed with 15mL oleic acid and 15mL octadecene, they were ad...

Embodiment 2

[0030] This embodiment is a NaYF-based 4 :Yb, the method for detecting thiamphenicol by the Er-gold nanoparticle fluorescent probe switch system is specifically completed according to the following steps:

[0031] 1. Detection of thiamphenicol standard solution: Take 500 μL and 2.5 mL of thiamphenicol standard solution with different concentrations (0.01 μmol / L~1 μmol / L) and add NaYF 4 :Yb, Er-gold nanoparticle fluorescent probe was incubated at room temperature for 15min, and the fluorescence spectrum of the solution after the addition of the above-mentioned different concentrations of thiamphenicol was recorded at 650nm using a steady-state transient fluorescence spectrometer to detect the excitation wavelength of 980nm the fluorescence intensity;

[0032] 2. Detection of thiamphenicol in egg samples: add a certain amount of thiamphenicol to 10 mL of evenly mixed egg liquid, dissolve it by ultrasonication, add 10 mL of trichloroacetic acid with a mass fraction of 10% to tre...

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Abstract

The invention discloses a thiamphenicol high-sensitivity detection method based on an up-conversion nanomaterial NaYF4: Yb, Er-gold nanoparticle fluorescent probe switch system, and relates to a thiamphenicol detection method. The invention aims to solve the problems that the existing thiamphenicol detection method is low in sensitivity and selectivity, some fluorescent materials excited by ultraviolet and visible wavelengths are relatively strong in background fluorescence in biological analysis and are not beneficial to analysis and detection of biological samples, and the like. The preparation method comprises the following steps: 1, preparing an oleate precursor; 2, preparing NaYF4: Yb, Er; 3, performing surface modification of NaYF4: Yb, Er; 4, preparing gold nanoparticles; 5, preparing the NaYF4: Yb, Er-gold nanoparticle fluorescent probe. The application method comprises the following steps: adding a thiamphenicol standard solution into an incubated NaYF4: Yb, Er-gold nanoparticle fluorescent probe solution, and carrying out fluorescence detection; adding the labeled and treated egg sample into an incubated NaYF4: Yb, Er-gold nanoparticle fluorescent probe solution, and carrying out fluorescence detection. According to the invention, the prepared NaYF4: Yb, Er-gold nanoparticle fluorescent probe is good in fluorescence performance. The established method is good in selectivity, low in detection limit and high in accuracy.

Description

technical field [0001] The invention belongs to the technical field of fluorescent nanomaterials and fluorescent sensing, and relates to an upconversion nanomaterial based on NaYF 4 : High sensitivity detection method of thiamphenicol in Yb,Er-gold nanoparticle fluorescent probe switch system. Background technique [0002] Thiamphenicol is a broad-spectrum antibacterial agent similar to chloramphenicol, which has played an important role in the treatment of human and animal diseases in recent years. Thiamphenicol can inhibit the protein synthesis of susceptible bacteria by binding to the ribosomal subunits in organisms, thereby preventing the transfer of amino acids and further formation of proteins, thereby achieving antibacterial effects. However, thiamphenicol bound to protein will produce a certain amount of free thiamphenicol if it stays in the body for a long time. etc.) excreted from the body. The antibiotics excreted by the human body and the residues of veterinar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/85C09K11/77
CPCC09K11/7773G01N21/6428
Inventor 牛娜衣佳琪陈立钢
Owner NORTHEAST FORESTRY UNIVERSITY
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