High-light-resistant Platymonas subcordiformis and application thereof
A technology of flat algae and light intensity, applied in the field of microorganisms, can solve problems such as damage, reduced photosynthetic efficiency, and difficulty in cultivating flat algae
Pending Publication Date: 2022-02-01
HAINAN UNIVERSITY
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Problems solved by technology
However, in southern regions such as Hainan, Guangdong, Fujian and other places, the solar altitude angle is relatively large, and the solar radiation obtained is relatively high, and the average light intensity in summer reaches 500 μmol·m -2 ·s -1 , far exceeding the level available for photosynthesis, excessive light will cause damage to the photosystem of microalgae, inhibit photosynthesis, and reduce photosynthetic efficiency
Method used
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Embodiment 1
[0036] 1. Collect the microalgae liquid from the aquaculture water in the surrounding sea area of Haikou City, and separate the relatively pure single algae colony (purity above 80%) through the plate marking and coating method, and the isolated microalgae MEM-A -412 was identified as a planella by ITS rRNA.
[0037] Wherein, the plate medium configuration is as follows:
[0038] Table 1 Nutrient solution mother solution formula (200mL)
[0039]
[0040] Table 2 vitamin mother solution formula (100mL)
[0041] Material name molecular formula level Weight (mg) Vitamin B12 Vitamin B12 AR 1 biotin Biotin AR 1 Vitamin B1 Vitamin B1 AR 20
[0042] Table 3 Trace element solution mother liquor formula (1L)
[0043] Material name molecular formula level Weight (g) Disodium edetate Na 2 EDTA
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The invention relates to the technical field of microorganisms, in particular to a strain of high-light-resistant Platymonas subcordiformis and application thereof. According to the invention, a strain of Tetramonas sp. MEM-A-412 is separated from a water sample and is preserved in the China Center for Type Culture Collection on August 25, 2021, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20211080. The algal strain can adapt to a highlight environment of 250 [mu] mol.m <-2 >. S <-1 > at most, can be widely used for cultivation in southern China, and provides a wide prospect for utilization of microalgae in southern China.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a flat algae resistant to high light and its application. Background technique [0002] Tetraselmis is a fast-moving single-celled green alga belonging to the genus Chlamydomonas of Chlamydomonas, Chrychophyceae, Volvoceae, Chlorophyceae, Chlorophyceae, and has a high lipid storage capacity and great potential for biodiesel oil production. In addition, long-chain w-3 fatty acids, namely docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are also produced. It can carry out photosynthetic oxygen release, has a fast reproduction speed, and is rich in nutrients. It is an excellent bait for fish, shrimp larvae and shellfish. Due to the special chemical composition and systemic position of Pyrophyta, it has attracted widespread attention from domestic and foreign scholars. At present, in the production of bait for aquaculture, the technology of artificially cultivatin...
Claims
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Login to View More IPC IPC(8): C12N1/12C12P7/649A23K50/80A23K10/16C12R1/89
CPCC12N1/12C12P7/649A23K50/80A23K10/16Y02E50/10
Inventor 路延笃杨琳
Owner HAINAN UNIVERSITY