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Method for producing wheat double haploid by distant hybridization of corn and wheat

A technology of double haploid and distant hybridization, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems that affect the breeding process, difficult to adapt, etc., and achieve improved breeding efficiency and low equipment operating costs , good resistance effect

Active Publication Date: 2022-02-15
襄阳市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing wheat haploid breeding technology is mainly to meet the pollen of normal autumn-sown wheat and winter greenhouse-grown corn at the flowering stage, and obtain DH plants after emasculation, pollination, hormone treatment, and chromosome doubling. Only one crop can be harvested a year. This affects the breeding process to a certain extent, and it is difficult to adapt to the current requirements for rapid breeding of new wheat varieties with high yield, high quality and multiple resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for producing double haploids of wheat by distant hybridization of corn and wheat, comprising the following steps:

[0031] The first step: In the experimental field of Xiangyang Academy of Agricultural Sciences, Hubei Province, in mid-April, organic fertilizers were applied to the experimental field. After the soil was improved, rake leveled and ditching was carried out. The sowing area of ​​each variety was 13.33 square meters, and the row spacing was 0.667. meters, the plant spacing is 0.3 meters, and the planting density is 3300 plants / mu for division, for use;

[0032] Check the artificial climate chamber to ensure that the temperature control, humidity control, and light intensity regulation functions in each section are good, and the ventilation effect is good. The artificial climate chamber is subjected to ultraviolet disinfection and ozone treatment one week before wheat planting, and it is ready for use;

[0033] Mix Dahan black peat soil (containing ...

Embodiment 2

[0045] A method for producing double haploids of wheat by distant hybridization of corn and wheat, comprising the following steps:

[0046] The first step: In the experimental field of Xiangyang Academy of Agricultural Sciences, Hubei Province, in mid-April, organic fertilizers were applied to the experimental field. After the soil was improved, rake leveled and ditching was carried out. The sowing area of ​​each variety was 13.33 square meters, and the row spacing was 0.667. meters, the plant spacing is 0.3 meters, and the planting density is 3300 plants / mu for division, for use;

[0047] Check the artificial climate chamber to ensure that the temperature control, humidity control, and light intensity regulation functions in each section are good, and the ventilation effect is good. The artificial climate chamber is subjected to ultraviolet disinfection and ozone treatment one week before wheat planting, and it is ready for use;

[0048] Mix Dahan black peat soil (containing ...

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Abstract

The invention belongs to the technical field of wheat breeding, and particularly relates to a method for producing wheat double haploids by distant hybridization of corn and wheat. The method comprises the following steps: planting wheat by using an intelligent artificial climate chamber, and adjusting the flowering phase of wheat to meet the flowering phase of normally growing corn; performing castration, pollination, hormone treatment, embryo rescue, seedling strengthening and chromosome doubling to obtain the wheat double haploid. The problem of flowering asynchronism of normally growing corn and wheat is solved. The method is matched with a traditional method for producing the wheat double haploid through distant hybridization of normal autumn sowing wheat and winter greenhouse planting corn, and two-season wheat double haploid materials with good resistance and high yield can be harvested in one year; the wheat breeding efficiency is greatly improved; wheat is planted in the phytotron, operation is easy, conditions are appropriate, and the equipment operation cost is low.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, and in particular relates to a method for producing wheat double haploid by distant hybridization of corn and wheat. Background technique [0002] Conventional wheat breeding technology needs to obtain homozygous and consistent stable new strains through parent selection, sexual hybridization, offspring segregation, artificial selection, gene homozygosity, and new strain identification. It takes 6 to 8 years for the offspring to start to segregate to gene homozygosity Time, the breeding process of a new strain takes about 10 years, which is time-consuming and labor-intensive. [0003] Wheat double haploid breeding is gradually being adopted by breeding experts and commercial breeding enterprises because it can speed up the wheat breeding process and shorten the breeding time of new wheat varieties. The existing wheat haploid breeding technology is mainly to meet the pollen of normal autum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01G22/20A01G24/15A01G24/28A01H1/08A01H4/00
CPCA01H1/021A01G22/20A01G24/15A01G24/28A01H1/08A01H4/008
Inventor 张小娟张鹏飞黄大明刘莹王立峰刘倩倩
Owner 襄阳市农业科学院