Method for simultaneous in-situ determination of enzymatic activity between straw and rhizosphere

A technology of enzyme activity and straw, applied in the field of soil microbiology, can solve the problems of inability to accurately distinguish in situ soil space, overestimation of soil enzyme activity, etc.

Pending Publication Date: 2022-02-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, commonly used soil enzyme activity determination methods cannot accurately distinguish in situ soil spatial differences, and most of these methods require destructive sampling of soil and plants, resulting in overestimation of soil enzyme activity

Method used

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  • Method for simultaneous in-situ determination of enzymatic activity between straw and rhizosphere
  • Method for simultaneous in-situ determination of enzymatic activity between straw and rhizosphere
  • Method for simultaneous in-situ determination of enzymatic activity between straw and rhizosphere

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Experimental program
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Effect test

Embodiment 1

[0043] The in situ assay method for enzyme activity in the stalk and rhizosphere under pot conditions comprises the following steps:

[0044] 1. Soil, plants, root box preparation

[0045] Remove stones and plant residues from the air-dried soil taken from the field and pass it through a 2mm sieve for later use. Gramineae crop stalks (wheat in particular) were selected as the research object. After the crops were harvested, the stalks were air-dried and crushed with a grinder, and passed through a 2mm sieve for later use.

[0046] 320 g of soil and 4 g of wheat straw (mass ratio 80:1) were mixed and loaded into custom-made root boxes. The root box is a flat cuboid, and the flat surface on one side can be opened. The movable surface is a transparent surface, which is convenient for observing the growth of the root system in the later stage, such as figure 1 . After mixing the soil and straw into the custom-made root box, wrap the root box with tinfoil and place it obliquely ...

Embodiment 2

[0053] The experimental soil was taken from a multi-year winter wheat-summer soybean model field in March 2019. The retrieved soil was manually removed from stones, plant residues and other impurities, air-dried, ground and passed through a 2mm sieve for later use. The wheat straw harvested in the summer of 2019 was selected as the crop straw. The wheat variety was Jimai 22. After the wheat straw was harvested, impurities such as soil clods were removed and then dried by a dryer. The above soil and wheat straw were all taken from the experimental field of Wuqiao Experimental Station of China Agricultural University. Maize was selected as the test plant, and the maize variety was "Zaohuangnuo". Soak the corn seeds to accelerate germination, and transplant when the root system grows 3cm from the seeds.

[0054] The inner length and width of the root box (potted plant) box used in this research are 12cm, and the inner thickness is 2cm. Take 360g of sieved air-dried soil and pu...

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Abstract

The invention discloses a method for simultaneous in-situ determination of enzymatic activity between straw and rhizosphere. The method comprises the following steps of: loading soil and straw into a root box, wherein the root box is wrapped with a light-proof material and obliquely placed on a support; transplanting plant seeds into the root box after germination, and after plants grow, exposing the plants out of soil surfaces and plant roots; meanwhile, taking a microporous filter membrane soaked in a substrate solution marked by fluorescent pigment, covering the surface of the microporous filter membrane, wrapping the root box with the light-proof material, and carrying out light-proof culture; after the culture is finished, taking down the microporous filter membrane, transferring the microporous filter membrane into an ultraviolet lamp box, and performing imaging under a 355nm reflection ultraviolet lamp; and processing and analyzing the obtained image, and comparing the image with a standard curve to obtain two-dimensional distribution of the enzymatic activity between the straw and the rhizosphere on the surface of the soil, namely simultaneously obtaining the enzymatic activity between the straw and the rhizosphere. According to the method, the in-situ enzyme activity determination between straw and rhizosphere under the control condition can be realized, destructive sampling of soil and plants is avoided, and the method is convenient and quick.

Description

technical field [0001] The invention relates to a method for simultaneously in situ measuring the enzyme activity of the stalk sphere and the rhizosphere, and belongs to the field of soil microbiology. Background technique [0002] Straw returning to the field is an important way of straw utilization, which has multiple functions such as increasing soil organic matter content, improving soil physical and chemical properties, increasing soil microorganisms, and changing soil enzyme activities. Among them, soil enzyme activity is a sensitive soil biological indicator. Soil enzymes refer to the accumulated enzymes in the soil, including hydrolytic enzymes, oxidases, transferases and lyases, which are mainly derived from the decomposition of soil microorganisms, plant root exudates and animal and plant residues. Enzyme is one of the most active organic components in soil components, and its activity can not only reflect the exuberant degree of energy metabolism of soil substanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34A01G22/00A01C1/00
CPCC12Q1/34A01G22/00A01C1/00G01N2333/942C12Q2334/22C12Q2334/20
Inventor 臧华栋徐茁王上周杰杨亚东曾昭海马千涵胡跃高
Owner CHINA AGRI UNIV
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