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Antibody diluent and preparation method thereof

A technology of diluent and antibody, applied in the field of antibody diluent and preparation, can solve the problems of poor signal, short storage time, high background, etc., and achieve the effect of enhancing immune detection signal, enhancing stability and ensuring versatility

Pending Publication Date: 2022-02-18
亚科因(武汉)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Skimmed milk powder cannot be used for phosphorylated proteins, the storage time is short, the color turns green, and new antibodies need to be replaced. Expensive antibodies are not suitable for use, so this type of antibody diluent is not very versatile.
Antibody diluents containing proteins such as BSA and serum are likely to cause cross-reactions, resulting in high background and poor detection signals

Method used

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  • Antibody diluent and preparation method thereof
  • Antibody diluent and preparation method thereof
  • Antibody diluent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This embodiment provides an antibody diluent, 1L of antibody diluent specifically includes the following components:

[0036] Polyethylene glycol 8000 53g, polyvinylpyrrolidone 40 1.6g, Tween-20 1mL, polysucrose 400 0.7g, ProClin300 preservative 1mL, trehalose 30g, TBS buffer solution (Tris -HCl buffer), the pH of TBS buffer is 7.2-7.6.

[0037] This embodiment also provides a method for preparing an antibody diluent, which specifically includes:

[0038] (1) Prepare 1L of 0.02mol / L TBS buffer solution, weigh 2.42g of Tris and 8g of NaCl and dissolve in 800mL of distilled water, adjust the pH to 7.2-7.6 with HCl, then add distilled water to make up to 1L.

[0039] (2) Take 800mL prepared TBS buffer solution, 53g of polyethylene glycol, 401.6g of polyvinylpyrrolidone, 4000.7g of polysucrose, 30g of trehalose, stir well, add Tween-20 1mL, ProClin300 preservative 1mL, Then dilute to 1 L with TBS buffer.

Embodiment 2

[0041] This embodiment provides an antibody diluent, 1L of antibody diluent specifically includes the following components:

[0042] Polyethylene glycol 8000 53g, Tween-20 1mL, ProClin300 preservative 1mL, trehalose 30g, the balance is 0.02mol / L TBS buffer (Tris-HCl buffer), the pH of TBS buffer is 7.2-7.6.

[0043] This embodiment also provides a preparation method of the antibody diluent, and the operation method is the same as that of the embodiment 1 except the dosage.

Embodiment 3

[0045] This embodiment provides an antibody diluent, 1L of antibody diluent specifically includes the following components:

[0046] Polyethylene glycol 8000 53g, polyvinylpyrrolidone 40 1.6g, Tween-20 1mL, ProClin300 preservative 1mL, trehalose 30g, TBS buffer (Tris-HCl buffer) with a balance of 0.02mol / L, TBS buffer pH is 7.2-7.6.

[0047] This embodiment also provides a preparation method of the antibody diluent, and the operation method is the same as that of the embodiment 1 except the dosage.

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Abstract

The invention provides an antibody diluent and a preparation method thereof. The antibody diluent is prepared from 40 g / L-65 g / L of a component A, 30 g / L-50 g / L of trehalose, 0.05%-0.1% by volume of a preservative, 0.05%-0.2% by volume of a surfactant and 0.015 mol / L-0. 025 mol / L of a Tris-HCl buffer solution with the pH value of 7-8, wherein the component A comprises polyethylene glycol. Compared with the prior art, the component A containing the polyethylene glycol is used for enhancing an immunodetection signal and enhancing the stability of the immunodetection signal, and the trehalose and the surfactant are used for reducing the background and enhancing the signal generated by immunodetection; and meanwhile, the Tris-HCl buffer solution is adopted, and the whole formula does not contain traditional substances such as protein and serum, so that the universality of antibodies in use is ensured.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to an antibody diluent and a preparation method. Background technique [0002] Immunoassay is a test method commonly used in life science research such as molecular biology, cell biology, biochemistry and immunogenetics. Experimental methods of factors, including enzyme-linked immunosorbent assay (Elisa), immunohistochemical assay (IHC), immunofluorescence assay (IFA), western blot assay (WB), co-immunoprecipitation assay (IP), flow cytometry, etc. . In immunoassays, the following problems are usually encountered: low abundance of the target protein or low antibody titer resulting in weak signal of the target protein band; poor blocking effect resulting in high background; poor antibody specificity resulting in non-specific bands Strong and other issues. Solving such problems usually requires a long period of condition optimization, especially changing antibodies or in...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/5306
Inventor 席永强柯珍叶健
Owner 亚科因(武汉)生物技术有限公司