Skin scaffold and preparation method thereof

Abstract

The invention belongs to the technical field of tissue engineering, particularly relates to a skin scaffold based on 3D biological printing, and provides a skin scaffold with anti-inflammatory, analgesic, cell viability protection and immune regulation functions. According to a technical scheme in the invention, the scaffold has a biological 3D printed three-dimensional grid structure and comprises the following components in percentage by weight: 10-35% of alginate, 60-85% of gelatin and 0.5-10% of paeoniflorin, preferably, a ratio of the weight of the paeoniflorin to the sum of the weights of the alginate and the gelatin is 0.03. A preparation method of the scaffold comprises the following steps: a, completely dissolving 0.5-10 parts by weight of paeoniflorin in 400-850 parts by weight of deionized water, adding 10-35 parts by weight of alginate powder and 60-85 parts by weight of gelatin particles, and conducting stirring at 30-50 DEG C for 0.5-4 hours to prepare biological 3D printing ink; b, adding the biological ink into a hot melting needle cylinder of a biological 3D printer, adjusting a constant temperature to 37 DEG C, inputting parameters of a grid needing to be formed into the printer, and printing a water-containing paeoniflorin-alginate-gelatin skin scaffold; and c, removing water from the water-containing skin scaffold in a negative temperature and vacuumizing environment.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a skin scaffold based on 3D bioprinting and a preparation method thereof. Background technique [0002] With the increasing number of diabetic patients, the incidence of diabetic foot ulcers is also increasing year by year. The treatment of diabetic foot ulcers has become a huge clinical challenge. Artificial skin plays an important role in the treatment of diabetic foot ulcers. In recent years In the past, bio-3D printing technology has developed rapidly, and personalized artificial skin scaffolds can be quickly obtained through this technology, which has gradually shown its unique advantages in the treatment of diabetic foot ulcers. However, the skin scaffolds obtained based on bio-3D printing technology are still Initial stage; [0003] The key to bio-3D printing technology is the selection and preparation of bio-ink. Currently, materials commonly used ...

AI Technical Summary

Problems solved by technology

[0002] With the increasing number of diabetic patients, the incidence of diabetic foot ulcers is also increasing year by year. The treatment of diabetic foot ulcers has become a huge clinical challenge. Artificial skin plays an important role in the treatment of diabetic foot ulcers. In recent years In the past, bio-3D printing technology has developed rapidly, and personalized artificial skin scaffolds can be quickly obtained through this technology, which has gradually shown its unique advantages in the treatment of diabetic foot ulcers. However, the skin scaffolds obtained based on bio-3D printing technology are still The initial stage;

[0003] The key to bio-3D printing technology is the selection and preparation of bio-ink. Currently, materials commonly used in bio-ink include: alginate, gelatin, collagen, chitosan, etc. These materials have their own advantages and disadvantages. For example, sodium alginate is A natural polysaccharide with high stability, solubility, viscosity and safety. Gelatin has high biocompatibility and degradability. It does not produce by-products and has no immunogenicity after degradation in the body. However, the starting point for the development of this type of skin scaffold is mainly the physical support of the scaffold and the degree of integration with human tissue, and there are still deficiencies in anti-inflammatory, analgesic, sedative, protective cell activity, and immune regulation.

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