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System for preparing sample solution for testing, and method for preparing sample solution for testing

A modulation system and sample technology, applied in the preparation of test samples, biochemical equipment and methods, specific-purpose bioreactors/fermenters, etc., can solve the problems of particle damage, component deterioration, etc., to prevent pollution, implement safety effect

Pending Publication Date: 2022-02-25
IHI CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in such an operation, there is a possibility that particle breakage due to centrifugal force and shear force applied to the sample, component deterioration due to turbulence, and foaming may occur (see the following non-patent document 2).

Method used

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  • System for preparing sample solution for testing, and method for preparing sample solution for testing
  • System for preparing sample solution for testing, and method for preparing sample solution for testing
  • System for preparing sample solution for testing, and method for preparing sample solution for testing

Examples

Experimental program
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Effect test

Embodiment 1

[0087] Referring to the particle separator described in the above-mentioned Patent Document 1, a flat plate shape with an arc-shaped curved flow path (rotational diameter: about 115 mm, radial cross-section of the flow path: rectangular, flow path width: about 3 mm) formed inside was produced. made of resin. This molded body was used as a channel unit to constitute a fluid force separation device. As the pump 10 for supplying the liquid, a syringe pump (repeatedly pushing and pulling the plunger to pressurize the fluid) is used, and the above-mentioned fluid force separation device is used to constitute image 3 Preparation System 1 of Sample Solution for Detection. However, the return path 8a is not provided, and the first fraction and the second fraction are recovered separately.

[0088] As a sample stock solution, yeast was added to water and dispersed to prepare an aqueous yeast solution containing yeast particles, and contained in the container 2 . Using a syringe pum...

Embodiment 2

[0093] Amino acid (L-alanyl-L-glutamine) and antibiotics (penicillin, streptomycin, amphoteric prime B). A CHO cell line (ATCC CRL-12445 Cricetulus griseus / CHO DP-12 clone #1932) was cultured in a liquid medium maintained at a temperature of 36.5 to 37.0°C. During the cultivation, the pH of the liquid medium was managed so that the pH would not become less than 6.70. The IgG antibody secreted by the cells was contained in the obtained culture solution, and its concentration was measured.

[0094] A system for preparing a sample solution for detection was configured in the same manner as in Example 1, and the cell culture solution obtained above was used as a sample stock solution and contained in the container 2 . Using a syringe pump, the cell culture solution in container 2 is pressure-fed to the curved flow path of the fluid force separation device at a constant flow rate. The first component on the outer peripheral side and the second component on the inner peripheral s...

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Abstract

A system for preparing a sample solution for testing is provided with a microorganism separation device (3) and can prepare a sample solution for testing from a sample stock solution. The microorganism separation device (3) is provided with a flow force separation device (4) and a liquid feed section (5) for feeding the sample stock solution to the flow force separation device (4). The flow force separation device (4) has a curved flow passage having a rectangular cross section, and can separate a liquid into a first fraction and a second fraction utilizing an eddying flow generated as the result of the flowing of the liquid through the curved flow passage. Particles contained in the sample stock solution is concentrated into the first fraction. It is possible to provide a sample solution for testing with which a test such as a microbiological test can be carried out efficiently and which can be subjected to the culture of a microorganism.

Description

technical field [0001] The present disclosure relates to a preparation system and a preparation method of a detection sample liquid for obtaining a detection sample liquid in a state suitable for detection from a sample stock solution for the purpose of detecting microorganisms and the like contained in the liquid. Background technique [0002] Usually, in the manufacture of pharmaceuticals, food and beverages, in order to manage the safety and quality of the products, microbial testing is carried out to detect the presence or absence of bacteria such as yeast and mold, as well as bacteria such as Escherichia coli and Legionella. For adequate and proper product stewardship, a large number of samples need to be tested. [0003] Conventionally, a culture method has been used for the detection of microorganisms, in which a liquid sample is used for culturing in an agar medium, and the number of colonies on the medium is measured as the bacterial survival number. However, it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40G01N1/10C12M1/34C12M1/00C12Q1/24
CPCG01N1/40G01N1/10C12Q1/24G01N1/4077G01N1/2035G01N2001/2064G01N2001/1012G01N15/0255G01N15/01G01N1/14G01N15/00C12M47/02C12M47/04C12N1/02
Inventor 池田谅介矶良行
Owner IHI CORP