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Hexapeptide with sleep improving activity and application thereof

A technology for improving sleep and activity, applied in hexapeptide with sleep-improving activity and its application fields, to achieve the effect of prolonging sleep time, increasing sleep rate, and increasing ratio

Pending Publication Date: 2022-03-01
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current research on food-derived sleep-improving active peptides is still insufficient.

Method used

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  • Hexapeptide with sleep improving activity and application thereof
  • Hexapeptide with sleep improving activity and application thereof
  • Hexapeptide with sleep improving activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Solid phase synthesis method synthesized hexapeptides to improve sleep activity:

[0048] 1 g of dichloro resin was swelling, washed, and the 9-fluorene methoxycarbonyl protecting group (FMOC protecting group) was added to the amino acid for condensation reaction, and the process of repeating the deprotection-condensation until all amino acids were joined. The resin was cut to obtain a hexapeptide crude to improve sleep activity, purified by reverse phase high performance liquid chromatography to give hexapeptide pure.

[0049] figure 1 Identification of hexapeptide Tyr-Val-Glu-Pro-Phe of Sleeping Activity for Liquid Phase-Mass Spectrometry. Depend on figure 1 It can be observed that the purified polypeptide pure product identification is hexapeptide Tyr-prop-Val-Glu-Pro-PHE.

Embodiment 2

[0051] (1) Caserhase hydrolysis: The casein and water are mixed with water, stir the water, heating to 37 ° C, first add 0.3% of the case of the casein, 37 ° C, enzymatic hydrolysis, lifting temperature to 95 ° C. Enzyme 15min To obtain an enzymatic hydrolyzate, 0.4% papain protease, 50 ° C is reduced to 95 ° C by 15 min, and the enzymatic hydrolyzate is filtered through filtration and spray drying to obtain case-proteinase.

[0052] (2) Separation of the obtained case-proteinase, separated from ethanol, and the gel filter column G25 was separated, and finally purified by the preparation stage UPLC to obtain 7.5-7.8 min, after mass spectrometry. To improve the hexapeptide of sleep activity.

Embodiment 3

[0054] (1) Caserin hydrolysis: Casein and water are stirred from 1: 12 to 1:12, the temperature is increased to 50 ° C, add 0.2% of the case with 0.2% of the trypsin directly to 2 0.5% papain, 50 ° C, 50 ° C, then heat up To 95 ° C for 15 min, the enzymatic hydrolyzate was filtered through filtration and spray drying to obtain caseophoside.

[0055] (2) Separation of the obtained case-proteinase, separated from ethanol, and the gel filter column G25 was separated, and finally purified by the preparation stage UPLC to obtain 7.5-7.8 min, after mass spectrometry. To improve the hexapeptide of sleep activity.

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Abstract

The invention discloses a hexapeptide with sleep improving activity and application of the hexapeptide. The primary structure amino acid sequence of the hexapeptide is Tyr-Pro-Val-Glu-Pro-Phe, and the molecular weight of the hexapeptide is 750.85 Da. The hexapeptide provided by the invention can be chemically synthesized, and also can be directionally prepared from casein enzymolysis products. The hexapeptide provided by the invention has obvious sleep improvement activity, which is mainly reflected in that the sleep rate of mice can be obviously increased and the time of pentobarbital sodium induced sleep of mice can be prolonged. Meanwhile, the ratio of the inhibitory neurotransmitter gamma-aminobutyric acid GABA to the excitatory neurotransmitter glutamic acid Glu can be remarkably increased, and the compound has a good effect of enhancing the sleep of the mice and can be directly used in food or medicine for preventing and treating insomnia or sleep disorder diseases.

Description

Technical field [0001] The invention belongs to the field of functional polypeptides, and in particular to a hexapeptide having improved sleep activity and its application thereof. Background technique [0002] Insomnia refers to a state in which it is difficult to fall asleep or difficult to keep sleep, including sleep latency, short sleep duration, more dream or awake. One-third of the people's life is sleep, the lack of sleep will lead to a series of issues, such as decline in life, fatigue, anxiety, memory, or even serious insomnia, will also cause depression and immune dysfunction. According to the latest investigation report of WHO in 2020, China's insomnia crowd has been as high as 36.1%. Insomnia is increasingly increasingly increasing the most serious problems, and has gradually become young in recent years, 90 young people have increasingly increasingly increasingly increasing problems such as night sleep, staying up late, stress, anxiety. At present, the drugs commonly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K14/47C12P21/06C07K1/04C07K1/22C07K1/16C07K1/14A61K38/08A61P25/20A23L33/18A23L2/52A23G3/36A23G3/44
CPCC07K7/06C07K14/4732C12P21/06A61P25/20A23L33/18A23L2/52A23G3/364A23G3/44A61K38/00A23V2002/00A23V2200/322A23V2250/5114A23V2250/642A23V2250/55A23V2250/038
Inventor 赵谋明钱兢菁郑淋赵翊君
Owner SOUTH CHINA UNIV OF TECH
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