Laser scanning super-resolution microscopic imaging device, method and equipment based on multiple signal classification algorithm and storage medium

Abstract

The invention discloses a laser scanning super-resolution microscopic imaging device, method and equipment based on a multi-signal classification algorithm, and a storage medium, belongs to the technical field of optical precision measurement, and aims to solve the problem that the transverse resolution of a confocal microscopic technology is difficult to improve. Comprising a laser light source, and a collimator objective, a scanning galvanometer, a telecentric scanning lens, a tube lens, a dichroscope, an objective lens, a fluorescence sample, an optical filter, a collection lens and a CCD camera are sequentially arranged in the light propagation direction of the laser light source. A CCD camera is used for collecting low-resolution image sequences generated in the random flickering process of a sample in different scanning focusing light spot illumination areas, a multi-signal classification algorithm is used for reconstructing the low-resolution image sequence of each scanning position, reconstruction results corresponding to all the scanning positions are spliced, and a high-resolution image is obtained. And the transverse resolution of the confocal microscopy technology can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of optical precision measurement, in particular to a laser scanning super-resolution microscopic imaging device, method, equipment and storage medium based on a multiple signal classification algorithm. Background technique [0002] In the field of live cell imaging, optical microscopy stands out among many methods due to its non-destructive and non-contact imaging properties, but the existence of the optical diffraction limit limits its imaging resolution. In order to realize the observation of smaller-sized cells and subcellular biological samples, it is of great significance to improve the imaging resolution of optical microscopy techniques. [0003] Confocal microscopy has unique advantages in axial tomography capability, but its lateral resolution is limited to 1.4 times that of ordinary wide-field microscopy, and its imaging results are greatly affected by the diameter of the pinhole, while existing im...

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Problems solved by technology

[0004] In order to overcome the defects in the above-mentioned prior art, the present invention provides a laser scanning super-resolution microscopic imaging device, method, equipment and storage medium based on a multiple signal classification algorithm, which can solve the problem that the horizontal resolution of confocal microscopy technology is difficult to improve

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