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Establishment method of two excellent gene fast transformation systems in quality improvement of strong gluten wheat

A method and technology for establishing wheat quality, which is applied in the field of establishing two excellent gene rapid transfer systems in the quality improvement of strong gluten wheat, and can solve the problems that research has not yet been carried out

Pending Publication Date: 2022-03-04
宋维富
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] At present, research on the directional transfer of high-quality glutenin subunits in spring wheat regions in Northeast China is limited to the genetic background of medium-strong gluten wheat varieties, and is a single-gene HMW-GS introduction. About HMW-GS and LMW-GS under the genetic background of strong gluten wheat Research on the method system of simultaneous introduction has not yet been carried out

Method used

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  • Establishment method of two excellent gene fast transformation systems in quality improvement of strong gluten wheat
  • Establishment method of two excellent gene fast transformation systems in quality improvement of strong gluten wheat
  • Establishment method of two excellent gene fast transformation systems in quality improvement of strong gluten wheat

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example 1

[0064] Example 1 Improves the Hybridization Mode of 2 Gene Introduction Efficiencies

[0065] When 6 times of selective backcrossing are used to simultaneously introduce 2 genes into the same variety, if each backcrossing generation selects a single plant carrying 2 genes for backcrossing, the backcrossing efficiency will be significantly reduced, because theoretically every The number of individual plants carrying two genes in a backcross generation population is only 1 / 4, which is a relatively small proportion. However, there may be no individual plants of this genotype in actual operation, and backcrossing cannot be performed. In the present invention, a single gene is selected for backcrossing, the ratio of positive individual plants is increased to 1 / 2, the number is doubled, and the backcrossing efficiency is significantly improved. After selective backcrossing of each gene for 6 times (the genetic background of the backcross parents reached 99.99%), positive individual ...

example 2

[0066] Example 2 Greenhouse Planting Accelerates Backcross Generation Process

[0067] Wheat is sown in spring in the Northeast spring wheat area, and it is ripe once a year. In order to speed up the backcrossing process, it is planted in the greenhouse for one season every year, and a selective backcrossing work is added. Practice shows that gene tracking and hybridization can be successfully completed in the greenhouse, and the backcrossing time can be shortened by half.

example 3

[0068] Example 3 Genomic DNA Extraction Method

[0069]The wheat genome was extracted using the CTAB method. Fresh wheat leaves were taken, ground to a fine powder in liquid nitrogen, and then transferred to a 1.5mL centrifuge tube. Add 700 μL of 2×CTAB extract preheated to 65°C (Tris-HCl 100mmol / L, EDTA 20mmol / L, NaCl 1.4mol / L, CTAB 2%), shake vigorously, and quickly put it into a 65°C water bath. Incubate for 1-2 hours (shake slowly once more than ten minutes), take out the centrifuge tube, add 350 μL of chloroform-isoamyl alcohol mixture (24:1), add 350 μL of Tris-balanced phenol, slowly invert the centrifuge tube 5-10 times, mix After homogenization, centrifuge at 13000 rpm for 18 min at 4°C. Take out the supernatant, put it into a new 1.5mL centrifuge tube, and add 350μL of chloroform-isoamyl alcohol mixture (24:1). Take out the supernatant, add 0.6 times the volume of ice-cold isopropanol, slowly invert the centrifuge tube until a flocculent precipitate appears, and pl...

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Abstract

The invention relates to a method for establishing a quick transformation system of two excellent genes in quality improvement of strong gluten wheat, which realizes directional tracking and transfer of genetic background LMW-GS of strong gluten wheat by utilizing a molecular marker technology, and establishes a quick transformation method for simultaneously introducing two excellent genes HMW-GS and LMW-GS in quality improvement of wheat. Results show that the gluten index, the Zeleny sedimentation value, the stabilization time, the stretching area, the extensibility and the maximum extension resistance are averagely improved by 10.7% and 12.5%, 7.3% and 9.1%, 28.7% and 47.4%, 5.5% and 38.1%,-5.9% and 5.2%, and 12.5% and 31.2% respectively by transferring the Glu-A3d and Glu-B1d genes, the positive effects of the Glu-A3d and Glu-B1d genes on the quality of the strong gluten wheat are verified, and the effect of improving the quality of the strong gluten wheat is obvious.

Description

technical field [0001] The invention relates to the technical field of plant variety improvement, in particular to a method for establishing a fast transfer system of two excellent genes in quality improvement of strong gluten wheat. Background technique [0002] "Marker-assisted backcross breeding" is a crop breeding method that combines marker-assisted selection technology with backcross breeding. In recent years, it has received widespread attention from crop breeders and pathologists in various countries, and has been widely used in breeding processes such as crop disease resistance (reverse) improvement and quality improvement (Huang, et al., 2002, Nature 418, 678- 684). Glutenin in wheat endosperm storage protein is the main component that determines the quality of wheat. It is divided into high molecular weight glutenin subunit (HMW-GS) and low molecular weight glutenin subunit (LMW-GS). GS) 2 parts. Zhang Yanbin, a researcher at the Institute of Crop Resources, He...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895
CPCA01H1/021A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 宋维富刘东军张延滨杨雪峰赵丽娟宋庆杰白光宇张春利辛文利肖志敏孙志玲孙雪松
Owner 宋维富
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