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Novel freshwater oil-producing microalgae species as well as culture method and application thereof

A cultivation method and aquatic technology, applied in the field of new freshwater oil-producing microalgae species and their cultivation, can solve the problems of large footprint, damage to the economy of microalgae energy production, high investment costs, etc., and achieve the goal of reducing cultivation costs Effect

Active Publication Date: 2022-03-11
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing culture technology, the yield of microalgae is not high, and the high-yield potential of microalgae is far from being brought into play, resulting in a large-scale cultivation area and high investment costs
The labor and materials consumed to maintain this culture system have seriously damaged the economics of microalgae energy production.

Method used

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  • Novel freshwater oil-producing microalgae species as well as culture method and application thereof
  • Novel freshwater oil-producing microalgae species as well as culture method and application thereof
  • Novel freshwater oil-producing microalgae species as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The algae cells were inoculated in BBM medium and cultured in a constant temperature and constant light incubator for 20 days. The culture conditions were: continuous light, and the light intensity was 100 μmol photons m -2 the s -1 , the temperature is 25°C. The results of algae culture were as follows: figure 2 shown.

Embodiment 2

[0049] The algae cells were inoculated in the BBM mixed medium containing 5wt% rice washing water, and cultivated in a constant temperature and constant light incubator for 20 days. The culture conditions were: continuous light, and the light intensity was 150 μmol photons m -2 the s -1 , the temperature is 20°C.

Embodiment 3

[0051] The algae cells were inoculated in the BBM mixed medium containing 10wt% rice washing water, and cultivated in a constant temperature and constant light incubator for 20 days. The culture conditions were: continuous light, and the light intensity was 100 μmol photons m -2 the s -1 , the temperature is 25°C. The results of algae culture image 3 shown.

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Abstract

The invention discloses a novel freshwater oil production microalgae species and a culture method and application thereof, the microalgae strain is Didymogenes chengda CDU-W13, the preservation time is December 3, 2021, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M20211536. The microalgae provided by the invention can effectively remove nitrogen, phosphorus and other substances in wastewater, can be used for treating wastewater, can be cultured in wastewater, and is low in culture cost; the microalgae strain disclosed by the invention has relatively high biomass yield (36 mg.L <-1 > day <-1 >) and grease content (46.3% dw), so that the microalgae strain has relatively high grease yield (16.67 mg.L <-1 > day <-1 >); the microalgae strain disclosed by the invention can be used for producing biodiesel, can also be subjected to large-scale culture, and is applied to wastewater treatment and production of other valuable compounds, such as production of grease, biodiesel, algal protein, fatty acid and the like.

Description

technical field [0001] The invention belongs to the technical field of microalgae, and in particular relates to a new freshwater oil-producing microalgae species and its cultivation method and application. Background technique [0002] Due to the energy crisis and global warming, coupled with human beings' emphasis on environmental protection, the world is forced to focus on finding new energy sources that can replace existing fossil fuels, and biofuels, as one of the alternative fuels, are considered to be the most promising. One of the promising alternatives. [0003] Among new energy sources, microalgae biodiesel is considered to be one of the most promising liquid green energy sources. Studies have shown that microalgal biomass has a higher oil content compared to other conventional oil feedstocks and thus can be used to produce advanced biofuels through refinery conversion processes. Moreover, the growth cycle of microalgae is short, and it can also be used in wastewa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/6463C12P7/649C12P7/40C12P21/00C02F3/34C12R1/89C02F103/32
CPCC12N1/12C12P7/6463C12P7/649C12P7/40C12P21/00C02F3/322C02F2103/32Y02E50/10
Inventor 黄进兰欣林玉玲郑林玲李丽马文标
Owner CHENGDU UNIV
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