Lactobacillus plantarum as well as product and application thereof
A technology of Lactobacillus plantarum and mycelium, applied in the field of microorganisms, can solve problems such as restricting healthy development, and achieve the effect of improving apparent digestibility and promoting growth
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1 strain screening, identification and preservation
[0029] 1. Screening
[0030] 1. Primary screening of Lactobacillus
[0031] The raw material of alfalfa was collected from Gongnong No. 5 alfalfa of Jilin Academy of Agricultural Sciences, and the raw material was cultured in an incubator at 37°C under anaerobic conditions for 2 days. Take 10g of the sample and put it in 90mL sterile water at 37°C and 180r / min shaker for 30min. Aspirate 1mL of the culture solution and dilute it to 10 with sterile saline -6 Take 100 μL of each dilution and spread it on the MRS solid medium, incubate anaerobically at 37°C for 24-48 hours, observe the growth of colonies on the medium, select a single colony, and repeatedly streak the microscopic examination on the MRS solid medium To pure species, store on a slant at 4°C. A total of 1 strain was isolated, numbered Z-11.
[0032] 2. Re-screening of Lactobacillus
[0033] 2.1 Bacteria culture
[0034] Inoculate 1 mL of the s...
Embodiment 2
[0065] The preparation of embodiment 2 probiotics
[0066] Inoculate strain Z-11 into MRS liquid medium and culture anaerobically in a 37°C incubator for 24 hours, take the fermentation liquid and centrifuge it at 7500r / min for 10min at 4°C, remove the supernatant, collect the bacteria and weigh them with sterile normal saline centrifuge again according to the above conditions, discard the supernatant, and wash twice repeatedly. Use 5 times (or 1:1 ratio) volume of protective agent (8g skimmed milk, 1g trehalose, 1.5g sodium glutamate, add 100g distilled water, sterilize at 115°C for 15min.) to resuspend the bacteria, stir for 30min, Pre-freeze in a -80°C refrigerator for 3 hours. When the temperature of the vacuum freeze dryer drops to -40°C to -50°C, the freeze-drying condition is a vacuum of 0.2mbar, and the freezer temperature is -55°C. Freeze-dry for 12-15h. The freeze-dried bacterial powder was evacuated by a vacuum packaging machine and stored at 4°C. After testing, t...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Viable count | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More