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Preparation method of multi-enzyme-containing efficient all-purpose membrane cleaning agent

A film cleaning agent, high-efficiency technology, applied in the direction of biochemical equipment and methods, detergent composition, detergent compounding agent, etc., can solve the problems of difficult storage of cleaning agents, affect the use, destroy the stability of enzyme preparations, etc., and achieve expansion Optimum temperature range, stable space, fast and convenient cleaning process

Pending Publication Date: 2022-03-18
杭州佳嘉乐生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the enzyme preparation is mixed with surfactants, additives, and solvents, the original stability of the enzyme preparation will be destroyed, making it difficult to preserve the cleaning agent, thus affecting the use

Method used

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  • Preparation method of multi-enzyme-containing efficient all-purpose membrane cleaning agent
  • Preparation method of multi-enzyme-containing efficient all-purpose membrane cleaning agent
  • Preparation method of multi-enzyme-containing efficient all-purpose membrane cleaning agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This embodiment provides a method for preparing a multi-enzyme-containing high-efficiency all-round membrane cleaning agent, comprising the following steps:

[0036](1) Preparation of glycosylase: Mix liquid acid protease, acid cellulase, α-amylase, pectinase, and lipase with glucosamine at a mass ratio of 2:1, and add 5 U / g of protein Transglutaminase. The reaction was stirred in a 25°C water bath for 6 hours to prepare the enzyme-glucose graft.

[0037] (2) Preparation of small molecule stabilizers by negative electrospray ionization and high-energy collision induction: VG-ZAB-2SE double-focusing mass spectrometer was used in the experiment. Hydrated deoxyribose samples were introduced into the spray needle via the Elite Pump 11 syringe motor at a flow rate of 3 μl / min. Needle voltages in the range of 5.15-5.66 kV allow for continuous jetting into an inlet capillary with an inner diameter of 750 μm. The inlet capillary was heated to 155-158°C to prevent clogging of...

Embodiment 2

[0041] This embodiment provides a method for preparing a multi-enzyme-containing high-efficiency all-round membrane cleaning agent, comprising the following steps:

[0042] (1) Preparation of glycosylase: Mix liquid acid protease, acid cellulase, α-amylase, pectinase, and lipase with glucosamine at a mass ratio of 3:1, and add 10 U / g of protein Transglutaminase. The reaction was stirred in a 35°C water bath for 3 hours to prepare the enzyme-glucose graft.

[0043] (2) Preparation of small molecule stabilizers by negative electrospray ionization and high-energy collision induction: VG-ZAB-2SE double-focusing mass spectrometer was used in the experiment. Hydrated deoxyribose samples were introduced into the spray needle via the Elite Pump 11 syringe motor at a flow rate of 4 μl / min. Needle voltages in the range of 5.15-5.66 kV allow for continuous jetting into an inlet capillary with an inner diameter of 750 μm. The inlet capillary was heated to 155-158°C to prevent clogging ...

Embodiment 3

[0047] This embodiment provides a method for preparing a multi-enzyme-containing high-efficiency all-round membrane cleaning agent, comprising the following steps:

[0048] (1) Preparation of glycosylase: Mix liquid acid protease, acid cellulase, α-amylase, pectinase, and lipase with glucosamine at a mass ratio of 4:1, and add 10 U / g of protein Transglutaminase. The reaction was stirred in a 45°C water bath for 1 hour to prepare the enzyme-glucose graft.

[0049] (2) Preparation of small molecule stabilizers by negative electrospray ionization and high-energy collision induction: VG-ZAB-2SE double-focusing mass spectrometer was used in the experiment. Hydrated deoxyribose samples were introduced into the spray needle via the Elite Pump 11 syringe motor at a flow rate of 5 μl / min. Needle voltages in the range of 5.15-5.66 kV allow for continuous jetting into an inlet capillary with an inner diameter of 750 μm. The inlet capillary was heated to 155-158°C to prevent clogging o...

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Abstract

The invention provides a preparation method of a multi-enzyme-containing efficient all-purpose membrane cleaning agent. The preparation method comprises the following steps: (1) preparing glycosylase; (2) negative spray ionization high-energy collision induction is carried out to prepare a small molecule stabilizer; (3) taking the following raw materials in percentage by mass: 6%-10% of the glycosylated acid protease prepared in the step (1), 6%-10% of acid cellulase, 6%-10% of alpha-amylase, 6%-10% of pectinase and 6%-10% of lipase; 1-3% of a micromolecular stabilizer prepared in the step (2); 5-10% of an ionic liquid; 0.5%-1.5% of a preservative; 10-20% of a complexing agent; and the balance of deionized water. And (4) preparing the membrane cleaning agent. According to the invention, the cleaning range of membrane pollutant types is expanded, and the cleaning effect of membrane pollution is effectively improved; the multi-enzyme cleaning and the ionic liquid auxiliary cleaning agent are cooperated, so that the damage to the membrane caused by multiple times of cleaning of the multi-flow and multi-type membrane cleaning agent can be reduced, the cleaning time can be greatly shortened, and the service life of the membrane is further prolonged.

Description

technical field [0001] The invention relates to the technical field of membrane cleaning agent development, in particular to a preparation method of a multi-enzyme-containing high-efficiency all-round membrane cleaning agent. Background technique [0002] The membrane technology developed in recent decades is based on the membrane material with selective permeability as the core, and the separation technology of mixture separation, purification and concentration is realized under the driving force of both sides of the membrane. Compared with traditional separation technologies such as filtration, rectification, extraction, and evaporation, membrane technology has the advantages of low energy consumption, high separation efficiency, simple equipment, no phase change, and no pollution, so it is called a new high-efficiency separation technology. In most industries in the 21st century, membrane technology will play an important role in the fields of water resources, energy, env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D3/386C11D7/26C11D7/32C11D7/60C12N9/18C12N9/20C12N9/24C12N9/42C12N9/50C12N9/88B01D65/06
CPCC11D3/38618C11D3/38627C11D3/38645C11D3/38636C11D3/38663C11D3/3869C11D7/3281C11D7/3245C11D7/268C11D7/265C12N9/50C12N9/2437C12N9/20C12N9/18C12N9/2402C12N9/88C12Y301/01011C12Y302/01015C12Y402/02002B01D65/02B01D2321/166
Inventor 陆跃乐孙雪高倩妮郑晓宇孔叶凯
Owner 杭州佳嘉乐生物技术有限公司
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