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Method for identifying gallnut tannin component based on UHPLC-ESI-QTOF-MS/MS

A UHPLC-ESI-QTOF-MS, gallic tannin technology, applied in material separation, analytical materials, measuring devices, etc., can solve the problems of long extraction time, cumbersome extraction process, etc., achieve low requirements, improve extraction and identification efficiency Effect

Pending Publication Date: 2022-03-18
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the current identification and analysis methods for gallic tannins often require cumbersome extraction procedures or a long extraction time, and fail to effectively identify gallic tannins with large molecular weight (above 1500Da)

Method used

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  • Method for identifying gallnut tannin component based on UHPLC-ESI-QTOF-MS/MS
  • Method for identifying gallnut tannin component based on UHPLC-ESI-QTOF-MS/MS
  • Method for identifying gallnut tannin component based on UHPLC-ESI-QTOF-MS/MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Extraction and identification of gallnut tannin components

[0037] 1.1. Extraction of gallnut tannin components

[0038] In this step, gallnut tannin components were extracted in the following three ways: high-pressure steam autoclave (121°C) extraction, ultrasonic-assisted water bath (60°C) extraction and ultrasonic-assisted water bath (65°C) extraction, specifically including the following operations:

[0039] 1.1.1. Use a sterilized mortar and grinding rod to directly grind the dried keratin samples, and crush the frozen fresh keratin samples with liquid nitrogen, and then grind them into fine powder. After grinding, pass through a 60-mesh sieve for homogenization, weigh 0.100g of the homogenized powder, then rinse it with a small amount of ultrapure water into a 100mL beaker, take 50mL of ultrapure water and add it to a 100mL beaker to obtain Galla galla powder slurry.

[0040] 1.1.2. The gallnut powder slurry in the beaker was subjected to the followi...

Embodiment 2

[0096] Embodiment 2: the extraction temperature selection of Chinese galla tannin composition

[0097] This embodiment extracts the tannin components in gallnut according to the method of ultrasonic-assisted water bath in embodiment 1, the only difference is that a series of temperature gradients (50°C, 55°C, 70°C, 75°C) are set in this embodiment ), as shown in Table 5 below. Subsequently, according to the UHPLC-ESI-QTOF-MS / MS identification technique in Example 1, the tannin component extracts of different temperature treatment groups were identified.

[0098] Table 5: Ultrasonic-assisted water-bath extraction of tannins in gallnut

[0099] group Extraction temperature (℃) Ultrasonic power (W) Extraction time (min) A 50 1500 30 B 55 1500 30 C 70 1500 30 D 75 1500 30

[0100] The results showed that 14 kinds of galloyl glucose (1-O-galloyl glucose to 14 galloyl glucose). However, the number of isomers identified for the 14 g...

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Abstract

The invention discloses a gallnut tannin component identification method based on UHPLC-ESI-QTOF-MS / MS, and belongs to the technical field of plant tannin identification. According to the identification method provided by the invention, the tannin component in the gallnut is extracted by using an ultrasonic-assisted water bath mode and the extracted tannin component is identified by using UHPLC-ESI-QTOF-MS / MS, so that 1-O-galloyl glucose to 14-O-galloyl glucose and a large number of isomers thereof in the tannin component in the gallnut can be identified in a broad spectrum; the effective identification of the high-molecular-weight tannin component in the gallnut tannin component is realized.

Description

technical field [0001] The invention belongs to the technical field of identification of plant tannins, in particular to gallic tannins based on ultra-high performance liquid chromatography-electrospray-quadrupole time-of-flight-mass spectrometry (UHPLC-ESI-QTOF-MS / MS) Method of identification of ingredients. Background technique [0002] Galla Chinensis is a gall formed by the metamorphosis of plant leaves caused by the parasitism of Galla Chinensis (Melaphis chinensis). According to the description of gallnut in the 2020 edition of "Pharmacopoeia of the People's Republic of China", Rhus Chinensis Mill, R. potaninii Maxim, R. punjabensis J.L.Stewart ex Brandis It is the source tree species of Galla. Gallnut can be divided into two types: Schlechtendalia Chinensis Bell and Schlechtendalia Chinensis Bell. The galls formed by Schlechtendalia Chinensis Bell parasitizing on the chinensis bell are called galls, which are important foreign exchange-earning forestry resources in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 张建国何彩云倪冰冰桑子阳
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY