Isothermal HIV nucleic acid detection kit and detection method

Abstract

The invention discloses an isothermal HIV nucleic acid detection kit and a detection method. The kit comprises a tube A reaction reagent and a tube B reaction reagent, the tube A reaction reagent comprises a walker and Protect chain, a trace single chain, a streptavidin magnetic bead and a buffer system containing NaCl; the B tube reaction reagent comprises gold a, gold b and a Mg < 2 + > buffer system. According to the kit, complex reagents and equipment are not needed in the detection process, laboratory environment operation can be avoided as much as possible, the result is presented in a visual mode, whether a detected object exists or not and the rough concentration range are visually and rapidly reflected, instant detection is achieved, and practical application is facilitated.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a rapid and convenient isothermal HIV nucleic acid detection kit and detection method. Background technique [0002] Detection of human immunodeficiency virus (HIV) is a key component of AIDS prevention and control programs. As a retrovirus, when it invades a cell, it reverse transcribes its own RNA into DNA and integrates it into the host cell genome. At present, the detection of HIV is mainly divided into nucleic acid detection and serological detection. The targets of serological detection are antigens or antibodies, commonly used methods include enzyme-linked immunosorbent assay (De la Rica and Stevens, 2012), immunofluorescence (Pandori et al., 2013) and Western blot (Zhou et al., 2015b), the existing test strips available on the market for personal testing generally use the ELISA method to detect HIV antibodies, but due to the low level of HIV anti...

AI Technical Summary

Problems solved by technology

[0004] Most of the results of existing HIV nucleic acid detection methods using DNA walkers are presented in fluorescence, but the observation of fluorescent signals requires certain equipment and cannot be displayed intuitively. It is even more difficult to operate outside the laboratory, and the cost of fluorescent groups is also high. It is difficult to quickly reflect the test results on the spot

Although the colorimetric analysis method based on gold nanoparticles is simple and low-cost, it can be used for on-site detection, but its sensitivity is low, which limits its clinical application in nucleic acid detection.

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