High-theaflavin instant black tea powder as well as in-vitro oxidation preparation method and application thereof
A technology for theaflavin and black tea powder, applied in the field of high-theaflavin instant black tea powder and its in vitro oxidation preparation, can solve the problems of rapid formation of unfavorable theaflavin, difficult process control, unstable enzyme activity and the like, and achieves perfection Nutritional structure of food, prolongation of food shelf life, and the effect of high theaflavin content
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[0035] The present invention also provides a method for in vitro oxidation preparation of the above-mentioned high theaflavin instant black tea, comprising the following steps:
[0036] (1) centrifuging the summer and autumn tea with hot water to obtain the tea extraction liquid;
[0037] (2) adding polyphenol oxidase and tannase to the tea extraction liquid in step (1) to ferment;
[0038] (3) separating the fermented liquid in step (2) through solid-liquid separation to obtain the liquid after the separation of the filtrate;
[0039] (4) drying the separated liquid in step (3) to obtain high theaflavin instant black tea powder.
[0040] In a preferred embodiment of the present invention, the temperature of the hot water extraction is 80-100°C, preferably, the solid-liquid ratio is 1:10-30, and more preferably, the hot water extraction time is 30-90min, more preferably, the conductivity of the leaching water is below 10 μS / cm.
[0041]In yet another preferred embodiment of...
Embodiment 1
[0053] The production steps of high theaflavin instant black tea powder are as follows:
[0054] (1) Tap water is purified through sand filter, carbon filter and reverse osmosis membrane in sequence, and the conductivity is monitored in real time to ensure that the conductivity of pure water is <10μS / cm;
[0055] (2) Take 10 kg of summer and autumn tea, extract with hot water according to the material-to-liquid ratio of 1:25, the extraction method is countercurrent extraction, and the extraction condition is extraction at 90°C for 60 minutes;
[0056] (3) The hot water extraction liquid is cooled and centrifuged to remove impurities to obtain a tea extraction liquid;
[0057] (4) Extracting and filtering the tea extraction liquid, adding polyphenol oxidase and tannase, and introducing sufficient air for in vitro fermentation. The amount of tannase added is 0.1% of the tea quality; the amount of polyphenol oxidase added is 0.5% of the tea quality; the fermentation temperature ...
Embodiment 2
[0061] The production steps of high theaflavin instant black tea powder are as follows:
[0062] (1) Tap water is purified through sand filter, carbon filter and reverse osmosis membrane in sequence, and the conductivity is monitored in real time to ensure that the conductivity of pure water is <10μS / cm;
[0063] (2) Take 15 kg of summer and autumn tea, extract with hot water according to the material-to-liquid ratio of 1:30, the extraction method is countercurrent extraction, and the extraction condition is to extract at 80°C for 90 minutes;
[0064] (3) The hot water extraction liquid is cooled and centrifuged to remove impurities to obtain a tea extraction liquid;
[0065] (4) Extracting and filtering the tea extraction liquid, adding polyphenol oxidase and tannase, and introducing sufficient air for in vitro fermentation. The amount of tannase added is 0.15% of the tea quality; the amount of polyphenol oxidase added is 0.55% of the tea quality, the fermentation temperatur...
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