Matrix-assisted laser desorption ionization-time-of-flight mass spectrometer and sample detection method

Abstract

The invention belongs to the technical field of detection, and particularly relates to a matrix-assisted laser desorption ionization-time-of-flight mass spectrometer and a sample detection method. The invention provides a matrix-assisted laser desorption ionization-time-of-flight mass spectrometer. The mass spectrometer comprises a vacuum system and an optical system, the optical system comprises a laser, a lens group and a reflecting mirror group, the reflecting mirror group in the optical system is replaced by a galvanometer system, and a light path of laser is adjusted by virtue of swinging of a galvanometer, so that accurate bombardment on a sample is further realized, the situation that ionization of the sample cannot be realized due to bombardment of an amorphous region is avoided, and the detection efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a matrix-assisted laser desorption ionization-time-of-flight mass spectrometer and a sample detection method. Background technique [0002] Matrix-assisted laser desorption ionization (Matrix-assisted laser desorption ionization, MALDI) was born more than 30 years ago, and has shown unique value in many disciplines and research fields. The laser performs soft ionization on the sample indirectly through the matrix, which well retains the macromolecules. Therefore, it has been more and more widely used in the research of biomacromolecules. The principle of matrix-assisted laser desorption ionization is to use laser irradiation to form a co-crystal between the sample and the matrix. The matrix absorbs energy from the laser, and energy transfer occurs between the matrix and the sample, so that the sample undergoes desorption ionization. The ionized sample acts in an ele...

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Problems solved by technology

The inhomogeneous distribution of molecules results in large signal variations from point to point at the time of detection, resulting in low detection efficiency with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)

[0004] At present, the laser introduction device of MALDI-TOF MS includes a laser, a reflector, and a lens group. The laser beam emitted by the laser enters the vacuum cavity formed by the sealed casing after passing through the reflector, and irradiates with the optical path at a certain angle to the bearing surface of the target plate. Into the vacuum cavity formed by the sealed shell, and then irradiate the sample through the lens group set in the sealed shell. The optical path of the laser is fixed. However, due to the "sweet spot" effect, there is a certain probability that the laser will hit In places without crystals, in order to obtain a good signal, it is necessary to continuously adjust the laser point. At present, the common way to adjust the laser point is to move the mechanical platform slowly. However, the moving speed of the mechanical movement platform is relatively slow, and it is difficult to match the high repetition frequency. Laser system, which can greatly increase analysis time and reduce research efficiency

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