Biological control method for wireworms in bamboo forest
A technology for needleworms and bamboo forests, applied in pest control, botany equipment and methods, chemicals for biological control, etc., can solve the problems of complicated treatment methods and cumbersome steps, and achieve good control effect and specificity Strong and good control effect
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Embodiment 1
[0030] The preparation of embodiment 1 metarhizium anisopliae granule
[0031] Take rice and vermiculite with a mass ratio of 5:10, and after autoclaving for 100 minutes, insert Metarhizium anisopliae. The inoculum amount of the liquid strain is 10% (mL / g). The water content is up to 70%, the amount of culture medium is 1 / 2 of the culture tank, and solid-state fermentation is carried out in a sterile constant temperature and humidity culture room at 25°C for 14 days. During this period, there is an important link, that is, the mycelium to be grown covers the fermentation medium Finally, it is necessary to shake the culture tank to fully disperse the mycelium, which can increase the spore production by 50%. The fermented product and light calcium carbonate were compounded at a ratio of 10:2 to obtain Metarhizium anisopliae granules, sealed with a vacuum sealing machine, and stored at 15°C.
[0032] After testing, the quality parameters of the Metarhizium anisopliae granule are...
Embodiment 2
[0033] The application of embodiment 2 metarhizium anisopliae granules
[0034] From late April to early May, 37.5kg / hm of Metarhizium anisopliae was applied during the plowing period of the bamboo forest 2 (ie 2.5kg / mu). The pesticide application method adopts furrow application, the furrow width is 20cm, the distance between the furrows is 50-80cm, and the furrow depth is 20-30cm. After the bacterial agent and sand are evenly mixed at a mass ratio of 1:5, they are applied in the furrow and then covered with soil. When the time comes, it can be mixed and applied together with the fertilizer; it will be applied again from early October to late October of the same year.
Embodiment 3
[0035] Example 3 Applying Metarhizium anisopliae granule and using chlorpyrifos granule control effect contrast
[0036]The test forest is located in Shanming Village, Deqing County, Huzhou City, Zhejiang Province, and the bamboo species is Zaoyuanzhu. Set up two different test control areas for the application of Metarhizium anisopliae granules and chlorpyrifos granules, each with an area of 20m × 20m, with no treatment blank as the control, and each treatment repeated 3 times. Each test area is separated by 5m. The method of administration of Metarhizium anisopliae granules is as described in Example 2. Chlorpyrifos granules are applied when plowing, and the application rate is 2.5kg / mu. Metarhizium anisopliae granules are applied continuously for three years, and chlorpyrifos granules are applied continuously for four years.
[0037] Before the experiment, during the period from mid-March to mid-April, regularly (every 7 days) the data on the damage rate of bamboo shoot...
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