Newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine containing immunopotentiator as well as preparation method and application of newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine

A dual inactivated vaccine and immune enhancer technology, applied in the field of immune adjuvant technology and biopharmaceuticals, can solve the problems of shortening the protection blank period and slow generation of immune protection, achieving long-lasting immune protection effect and improving bioavailability the effect of reducing toxicity and side effects

Pending Publication Date: 2022-04-08
浙江洪晟生物科技股份有限公司 +2
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with live vaccines, oil emulsion inactivated vaccines produce slower immune protection. Therefore, it is necessary to develop vacci...

Method used

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  • Newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine containing immunopotentiator as well as preparation method and application of newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine
  • Newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine containing immunopotentiator as well as preparation method and application of newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine
  • Newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine containing immunopotentiator as well as preparation method and application of newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine

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example 1

[0077] 1. Experimental materials

[0078] The inactivated Newcastle disease virus liquid and the inactivated avian influenza H9N2 subtype virus liquid are inactivated by 1% formaldehyde solution.

[0079] 14-day-old healthy white-feathered broiler chickens.

[0080] 2. Preparation of the vaccine

[0081] Weigh 10 mg of polymyocytes, dissolve them in 1 ml of normal saline, and make No. No. 2 mother liquor of 20mg / ml. All filter sterilized.

[0082] The immune enhancer, inactivated Newcastle disease virus liquid, inactivated avian influenza H9N2 subtype virus liquid and Tween-80 (Tween 80) were prepared according to the volume ratio of 2: 1.88: 1.8: 0.24 water phase solution, added Tween Immediately after that, put in a water bath for about 5 minutes, shake to mix well. The volume ratio of Span-80 and white oil was 1:19, and 0.06 mg of aluminum stearate was added to prepare the oil phase. Stir for 5 minutes by a high-speed emulsifier at 13000 rpm / min to obtain experime...

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Abstract

The invention discloses a newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine containing an immunopotentiator as well as a preparation method and application of the newcastle disease and H9 subtype avian influenza bivalent inactivated vaccine, the immunopotentiator contains 1-1000 [mu] g/ml of polyinosinic acid and 1-1000 [mu] g/ml of beta-cyclodextrin, and the immunopotentiator, an inactivated antigen and Tween are mixed to prepare a water-phase solution; mixing white oil, span and aluminum stearate to obtain an oil phase solution; and mixing the oil-phase solution with the water-phase solution to obtain the inactivated vaccine. The immunopotentiator contained in the inactivated vaccine and other components in the vaccine have positive guidance quality; the preparation has the advantages of increasing the solubility, dissolution rate and stability of vaccine components, controlling the release rate of the vaccine, improving the bioavailability of the vaccine, reducing toxic and side effects, starting an immune response program contained in an organism and the like, so that the antibody level of the immunized organism is improved, and the immune effect is enhanced; after immunization, the chicken antibody response level is obviously enhanced, the capability of generating high antibodies is achieved, the immune effect is good, and the immune protection acting force is lasting and long.

Description

technical field [0001] The present invention relates to a dual inactivated vaccine and its preparation method and application, more specifically, it relates to a dual inactivated vaccine containing Newcastle disease and H9 subtype avian influenza containing an immune enhancer, as well as its preparation method and application, belonging to the immune adjuvant vaccine. Drug technology and biopharmaceutical field. Background technique [0002] Newcastle disease (ND) is caused by Newcastle disease virus (NDV) infection of the genus Paramyxovirus in the family Paramyxoviridae, causing rapid high fever, exhausted feed intake, mental disorders, dyspnea, yellow and white diarrhea , Lethargy is a highly contagious infectious disease characterized by lethargy. The disease can spread throughout the year and can harm chickens of any age and variety. Its high morbidity and mortality have caused great harm to the poultry farming industry. The World Organization for Animal Health (Office...

Claims

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Application Information

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IPC IPC(8): A61K39/17A61K39/145A61K39/39A61P31/14A61P31/16
Inventor 冯华朋舒建洪张金鸽张兴赵镓烨何玉龙查银河
Owner 浙江洪晟生物科技股份有限公司
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