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HPLC fingerprint spectrum detection method for Tibetan codonopsis pilosula

A technology of fingerprints and detection methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of chemical identification method research on the distinction between Tibetan Codonopsis pilosula and Tibetan Codonopsis pilosula, and the distinction between gray-haired Codonopsis pilosula and veined Codonopsis pilosula in Tibetan Codonopsis pilosula And other issues

Pending Publication Date: 2022-04-12
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there are few studies on the quality of Tibetan Codonopsis, and there is no research on the chemical identification method to distinguish Codonopsis Codonopsis from Tibetan Codonopsis, and there is no report on the distinction between gray hairy Codonopsis and veined Codonopsis Codonopsis in Tibetan Codonopsis

Method used

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  • HPLC fingerprint spectrum detection method for Tibetan codonopsis pilosula
  • HPLC fingerprint spectrum detection method for Tibetan codonopsis pilosula
  • HPLC fingerprint spectrum detection method for Tibetan codonopsis pilosula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Fingerprint detection of Codonopsis pilosula of the present invention

[0038] 1) Preparation of reference solution

[0039] Take chlorogenic acid, luteolin, luteolin and apigenin reference substances, weigh them accurately, add 50% ethanol to make a mixed reference solution with a mass concentration of each component of 0.1 mg / mL.

[0040] 2) Preparation of the test solution

[0041] Weigh 1 g of the aerial part of Codonopsis pilosula (passed through a No. 4 sieve), weigh it accurately, put it in a stoppered Erlenmeyer flask, add 25 mL of 50% ethanol accurately, weigh it, and ultrasonically treat it (power 100W, frequency 45kHz) for 45min , then weigh the mass, make up the lost mass with 50% ethanol, shake well, filter, and take the filtrate to obtain the final product.

[0042] 3) Accurately draw 10 μl each of the reference substance solution and the test solution, inject them into the liquid chromatograph, and record the chromatograms. The chromatographi...

Embodiment 2

[0046] Embodiment 2 Fingerprint detection of Codonopsis pilosula of the present invention

[0047] 1) Preparation of reference solution

[0048] Take chlorogenic acid, luteolin, luteolin and apigenin reference substances, weigh them accurately, add 50% ethanol to make a mixed reference solution with a mass concentration of each component of 0.2 mg / mL.

[0049] 2) Preparation of the test solution

[0050] Weigh 2 g of the medicinal material powder of Codonopsis pilosula (through No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 80 mL of 50% ethanol, weigh it, and ultrasonically treat it (power 100W, frequency 45kHz) for 45min , then weigh the mass, make up the lost mass with 50% ethanol, shake well, filter, and take the filtrate to obtain the final product.

[0051] 3) Accurately draw 10 μl each of the reference substance solution and the test solution, inject them into the liquid chromatograph, and record the chromatograms. The chroma...

experiment example 1

[0057] 1 material

[0058] 1.1 Instrument

[0059] UItiMate3000 high performance liquid chromatograph (Thermo Fisher Scientific China Co., Ltd.); BP121S 1 / 100,000 electronic analytical balance (company); Milli-Q ultrapure water machine; KQ-500VDE dual-frequency numerical control ultrasonic cleaner (Kunshan City Ultrasonic Instrument Co., Ltd.); DZKW-4 electronic constant temperature water bath (Beijing Zhongxing Weiye Instrument Co., Ltd.).

[0060] 1.2 Reagent

[0061] Reference substances chlorogenic acid (batch number MUST-21030304), luteolin (batch number MUST-20062810), luteolin (batch number MUST-20102418), apigenin (batch number MUST-21030615) were purchased from Chengdu Master Biotechnology Co., Ltd. company, the mass fraction is greater than 98%; acetonitrile is chromatographically pure; water is ultrapure water; others are analytically pure.

[0062] 18 batches of gray and hairy Codonopsis, 17 batches of Maihua Codonopsis, 2 batches of Codonopsis pilosula, 1 batch...

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Abstract

The invention discloses an HPLC (High Performance Liquid Chromatography) fingerprint spectrum detection method of Tibetan codonopsis pilosula. The method comprises the following steps: 1) preparing a reference substance solution; 2) preparing a test solution; and (3) respectively sucking the reference substance solution and the test solution, injecting into a high performance liquid chromatograph, and recording chromatograms. According to the method disclosed by the invention, the fingerprint spectrum of the Tibetan codonopsis pilosula is established for the first time through a specific sample treatment method and chromatographic conditions, and four peaks, namely chlorogenic acid (peak No. 2), luteoloside (peak No. 4), luteolin (peak No. 6) and apigenin (peak No. 7), are identified through comparison of reference substances. The invention provides a method for detecting chlorogenic acid, galuteolin, luteolin and apigenin in the Tibetan codonopsis pilosula, and also provides a scientific basis for quality evaluation and clinical application of the Tibetan codonopsis pilosula.

Description

technical field [0001] The invention relates to an HPLC fingerprint spectrum detection method of Tibetan Codonopsis pilosula. Background technique [0002] Codonopsis canescens Nannf. and C.foetens subsp.nervosa (Chipp) D.Y.Hong are commonly used Tibetan medicines, and they are the local mainstream species of the Codonopsis genus of Campanulaceae. Tibetan medicine is commonly called Tibetan Codonopsis, mainly distributed in Sichuan Province, In Qinghai Province and Tibet Autonomous Region, it is recorded in "Tara Materia Medica" and "Jingzhu Materia Medica". Its sources also include long-flowered Codonopsis C.thalictrifoliavar.Mollis, Codonopsis C.pilosula, etc., which have the effects of reducing inflammation and swelling, expelling wind and dampness, strengthening the spleen and stomach, and invigorating Qi. It is used to treat rheumatoid arthritis, leprosy, eczema, yellow Water sickness, beriberi and other diseases. [0003] Dangshen CODONOPSIS RADIX is sweet and flat i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCY02A50/30
Inventor 蒋桂华李凤超李惠敏高必兴袁茂华杜华范刚古锐刚焕晨雷连艳尹显梅艾莉郭佳晨
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE