Novel peptonia solani strain and application thereof
A technology of Solon stripping tube fungus and strains, which is applied in the cultivation and application fields of edible and medicinal fungi, can solve the problems of loss of benefits, aggravation of environmental pollution, and ineffective use of nutrient resources, and achieve the realization of recycling of waste resources, The effect of broad application prospects
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Embodiment 1
[0034] The isolation and identification of embodiment 1 Solon's tube fungus
[0035] 1. Sample collection and habitat survey
[0036] Use the GPS positioning method to obtain the sample distribution area and accurate geographical location, and investigate the habitat conditions such as landform type, altitude, temperature, and precipitation in the area:
[0037] L-DKJ( figure 1 ) was collected at Xiaowangliuhe Village, Lulong County, Qinhuangdao City, Hebei Province, with longitude E118.8994, latitude N39.8980, and an altitude of 55 meters; the collection time was July 15, 2019. Lulong County belongs to the warm temperate continental monsoon climate zone, with four distinct seasons, the annual average temperature is 10.7°C, and the precipitation is 725cm, with abundant rainfall;
[0038] YJY-88 was collected in Yangjiayu Village, Qianxi County, Tangshan City, Hebei Province, longitude E118.1132, latitude N40. 2122, and altitude 167 meters; the collection time was August 20, ...
Embodiment 2
[0066] Example 2 Screening and Optimization of Original Seed, Cultivated Species, and Cultivation Compost Formula
[0067] 1. Screening of the original seed compost formula
[0068] Set 5 groups of original seed compost formulas, the weight percentages are as follows:
[0069] A. Chestnut shell 98%, gypsum 2%;
[0070] B. Chestnut twigs 48%, chestnut shell 35%, bran 15%, sucrose 1%, gypsum 1%;
[0071] C. Chestnut twigs 48%, chestnut shell 30%, bran 20%, sucrose 1%, gypsum 1%;
[0072] D. Chestnut twigs 48%, corn cob 30%, bran 20%, sucrose 1%, gypsum 1%;
[0073] E. Chestnut shell 88%, bran 10%, gypsum 2%.
[0074] According to the above formula, prepare the required composts such as chestnut twigs (twigs under pruning, silky), chestnut shells, corn cobs and so on and weigh them accurately.
[0075] In the process of mixing materials, first add various main materials such as chestnut twigs, chestnut shells, and bran into the paddle mixer evenly, and then perform a mechani...
Embodiment 3
[0114] Embodiment 3 volatile component analysis
[0115] Prepare L-DKJ and YJY-88 solid culture (mycelium), liquid fermentation product, solid fermentation film (old bacterial skin) and fruiting bodies, and detect their volatile components respectively:
[0116] (1) Solid culture (aerial mycelium): Pour about 5cm of PDA solid medium into a 15mL headspace bottle in a clean bench, insert L-DKJ strains with a diameter of 5cm, and store at 25°C. Dark and constant temperature culture for 21 days;
[0117] (2) Liquid fermentation product: insert 3 fungus cakes with a diameter of 5 cm into the PDA liquid medium in the ultra-clean workbench, and culture on a constant temperature shaking shaker at 25°C in complete darkness and 120r / min for 21 days;
[0118] (3) Solid fermentation bacteria film (old bacteria skin): take the completely overgrown original seed / cultivated species bottle mouth surface layer bacteria membrane (old bacteria skin), remove surface impurities, and after natural...
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