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Preparation method and fermentation device of gamma-aminobutyric acid

A technology of aminobutyric acid and fermentation equipment, applied in biochemical equipment and methods, fermentation, bioreactors/fermenters for specific purposes, etc., can solve the problems of affecting fermentation efficiency, poor stirring effect, and poor fermentation products, etc. problems, to achieve the effect of improving fermentation efficiency, shortening fermentation cycle and reducing dosage

Inactive Publication Date: 2022-04-15
枣庄市杰诺生物酶有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to: provide a preparation method of γ-aminobutyric acid and its fermentation device, to solve the above-mentioned background technology that the fermented product cannot be in good contact with the air, and the stirring effect is not good, which will affect the overall The problem of fermentation efficiency

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  • Preparation method and fermentation device of gamma-aminobutyric acid
  • Preparation method and fermentation device of gamma-aminobutyric acid
  • Preparation method and fermentation device of gamma-aminobutyric acid

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Embodiment Construction

[0029] In order to make those skilled in the art better understand the solutions of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only The embodiments are part of the present application, but not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the scope of protection of the present application.

[0030] It should be noted that the terms "first", "second", etc. in the description and claims of the present application and the above drawings are used to distinguish similar objects, and are not necessarily used to describe a specific sequence or sequence. It is to be understood that the data so used are interchangeable un...

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Abstract

The invention discloses a preparation method of gamma-aminobutyric acid and a fermentation device thereof, and relates to the technical field of gamma-aminobutyric acid preparation, and the method comprises the following steps: inoculating a cultured gamma-aminobutyric acid production strain into a fermentation medium, putting the fermentation medium into the fermentation device, and carrying out aerobic and anaerobic two-stage combined fermentation to obtain the gamma-aminobutyric acid. The first stage is an aerobic fermentation stage, when the cell concentration reaches 15-30, a second stage of anaerobic fermentation is carried out, anaerobic culture is carried out until fermentation is finished, and gamma-aminobutyric acid is obtained; the fermentation device comprises a tank body, and further comprises a stirring mechanism for promoting fermentation of fermented materials, the stirring mechanism comprises a rotating assembly, a deflection assembly and a gas injection assembly. On the premise that the number of the stirring rods is not increased, the stirring area is increased, the resistance during stirring, namely the resistance borne by the stirring rods during stirring, is reduced, the stirring is performed in the transverse direction and the longitudinal direction, and the fermentation efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of preparation of γ-aminobutyric acid, in particular to a preparation method of γ-aminobutyric acid and a fermentation device thereof. Background technique [0002] Gamma-aminobutyric acid (referred to as GABA), also known as 4-aminobutyric acid, γ-aminobutyric acid, the molecular formula is C 4 H 9 NO 2 , relative molecular weight 103.2, is a white or nearly white crystalline powder, easily soluble in water, slightly soluble in hot ethanol, insoluble in cold ethanol, benzene, ether, melting point (mp): 203-204 ℃ (decomposition), Melt and decompose into pyrrolidone and water. [0003] Due to the low natural presence of GABA, it is difficult to extract and isolate a large amount of GABA from some natural tissues. At present, the methods for obtaining GABA are chemical synthesis and biological methods. Biological methods include enrichment in plants and microbial fermentation. The microbial fermentation me...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/06C12M1/00C12P13/00
Inventor 许向阳张艳侠宋在伟王鹏宋均营黄家超
Owner 枣庄市杰诺生物酶有限公司
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