Acute coronary syndrome symptom diagnosis model construction and application method

Abstract

The invention discloses an acute coronary syndrome symptom diagnosis model construction and application method. The construction method comprises the following steps: collecting a blood sample and clinical data of a patient suffering from chest pain in hospital admission, centrifuging the blood sample by using an EDTA (Ethylene Diamine Tetraacetic Acid) anticoagulation tube, and separately storing a plasma sample obtained after centrifugation in a refrigerator at the temperature of-80 DEG C; the method comprises the following steps: carrying out protein precipitation on a plasma sample, oscillating, centrifuging, taking a supernatant for sample introduction, separating 12 ceramides in the plasma sample by using an ultra-high performance liquid chromatography system, establishing a calibration curve by using a mass spectrometer through a mass spectrum isotope internal standard quantification method, and detecting 12 ceramide molecule concentration values of all plasma samples; fitting a linear model of a logistic regression method based on traditional clinical variables of a patient with chest pain and troponin and ceramide data, and calculating optimal model parameters to obtain an acute coronary syndrome symptom diagnosis model; based on the acute coronary syndrome symptom diagnosis model, whether the patient is an ACS patient can be rapidly judged, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of medical diagnostic models, in particular to a construction and application method of a diagnostic model of acute coronary syndrome. Background technique [0002] Acute coronary syndrome (ACS) is a group of clinical syndromes based on the rupture or invasion of coronary atherosclerotic plaques, followed by complete or incomplete occlusive thrombosis. Recent reports show that about 1.4 million people in the United States get sick every year, and this number is as high as 2.5 million in China. About 30% of the population may be exposed to the risk of ACS in their lifetime. [0003] Blood circulation biomarkers play an important role in the establishment of diagnosis, treatment decision and prognosis evaluation of ACS. However, the current traditional biomarkers also have aspects to be perfected and improved. First, traditional coronary artery disease risk factors do not adequately distinguish patients sus...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a diagnostic model construction and application method for acute coronary syndrome, so as to solve the problem that there is no method for constructing a diagnostic model for diagnosing acute coronary syndrome based on ceramide at present in the industry, which limits the research found in practical application problems

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