Unlock instant, AI-driven research and patent intelligence for your innovation.
Polyphenol-mediated multifunctional bionic metal-organic framework mixed structure as well as preparation and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A reaction and nanoparticle technology, applied in organic active ingredients, biochemical equipment and methods, medical preparations of non-active ingredients, etc., can solve problems such as low tumor selectivity, weakened cancer treatment effect, and unsatisfactory PDT treatment effect , to achieve the effect of broad application prospects
Pending Publication Date: 2022-04-19
HEBEI UNIV OF TECH
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
In addition, the hypoxic environment and continuous oxygen consumption during PDT will further weaken the therapeutic effect on cancer
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0032] Example 1, Preparation of L-Arg@PCN@GOx-TA@Mem Nanoparticles (mPAGT NPs)
[0033] First, ZrOCl 2 ·8H 2 O (150 mg, 0.465 mmol), TCPP (50 mg, 0.065 mmol) and BA (1.4 g, 11.5 mmol) were dissolved in 50 mL of DMF. The mixture was then gently stirred at 90°C in the dark for 5 hours. Finally, PCN-224 (PCN) nanoparticles were purified by centrifugation (15000 rpm) for 20 min, and then washed 3 times with DMF. L-Arg (10 mg) was added to PCN-224NPs (1 mg / mL, 10 mL) aqueous solution. After stirring for 24 h at room temperature in the dark, PA NPs (L-Arg@PCN) were centrifuged (15,000 rpm) for 20 min, and then washed with water three times. Finally, the product was redispersed in water and stored in a refrigerator at 4°C, protected from light. TA (4mg) was added to the obtained PA NPs (2.5mg / mL, 5mL) solution, stirred at 37°C for 0.5h, and then GOx (6mg) was added. Stirring was continued at 37°C for 3.5 h, and PAGT NPs (L-Arg@PCN@GOx-TA) were collected by centrifugation and s...
[0039] The PCN@GOx-TA NPs used in the following experiments, namely PGT NPs, were prepared by the following method:
[0040] Add TA to PCN NPs aqueous solution, stir and react at 37°C for 0.5h, then add GOx, and continue stirring at 37°C for 3.5h to obtain PCN@GOx-TA NPs, namely PGT NPs;
[0041] The PCN@Mem NPs, L-Arg@PCN@Mem NPs and PCN@GOx-TA@Mem NPs used in the following experiments, i.e. mPCNNPs, mPA NPs and mPGT NPs, were prepared by the following methods:
[0042] The PCN NPs, PA NPs and PGT NPs were encapsulated with the cell membrane of cancer cells to be targeted to obtain mPCN NPs, mPANPs and mPGT NPs.
[0043] Cell membrane extraction method, first, mouse breast cancer (4T1) cells were incubated, collected, suspended in PBS for 2 minutes, centrifuged (5000 rpm, 4 minutes), then added water, crushed with a cell disruptor, and then crushed The 4T1 cells were centrifuged at 8000rpm for 5min, and the supernat...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a polyphenol-mediated multifunctional bionic metal-organic framework mixed structure as well as preparation and application thereof. The invention relates to a nano-particle, namely, L-Arg (at) PCN (at) GOx-TA (at) Mem nano-particle, L-Arg represents L-arginine, PCN represents MOFs (Metal-Organic Frameworks) prepared by taking ZrOCl2, meso-tetra (4-carboxyphenyl) porphin (TCPP) and benzoic acid (BA) as raw materials to react, GOx-TA represents glucose oxidase-tannic acid, and Mem represents a cell membrane of a cancercell to be targeted. The nano particles realize cascade synergistic interaction of a starvation therapy, a photodynamic therapy and a gas therapy to treat tumors / cancers.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a photofunctional nanomaterial capable of catalyzing cascade enhanced hunger / photodynamic / gas synergistic therapy and a preparation method thereof, more specifically to a polyphenol-mediated multifunctional biomimetic metal -Organic framework hybrid structure and its preparation method and its application in the preparation of drugs for tumor-targeted combination therapy, with polyphenol-mediated coating strategy, catalytic cascade enhanced tumor-targeted combination therapy, good homologous targeting ability and Good colloidal properties. Background technique [0002] Photodynamic therapy (PDT) is an emerging photochemical-based approach to cancer treatment. When irradiated with specific light, the nontoxic photosensitizer is activated and transfers the energy of the light to the surrounding oxygen molecules, inducing a series of cytotoxic reactive oxygen species. In recent ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More