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Monitoring device and monitoring method for growth of bacteria/cells

A monitoring device and bacteria technology, applied in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of lack of physiological environment bacteria/cell growth monitoring, response ability, etc., and achieve improved conductivity and biocompatibility improved effect

Pending Publication Date: 2022-04-29
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the bottleneck problem of lack of monitoring and response ability of bacteria / cell growth in physiological environment in existing titanium-based implant devices, and to provide a monitoring device and monitoring method for bacteria / cell growth

Method used

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  • Monitoring device and monitoring method for growth of bacteria/cells
  • Monitoring device and monitoring method for growth of bacteria/cells
  • Monitoring device and monitoring method for growth of bacteria/cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Pretreatment: Pickling the titanium sheet, during pickling, the pickling solution is composed of HF with a mass fraction of 40%, and HNO with a mass fraction of 60%. 3 Mixed, pickling time is 10min; HF, HNO 3 The volume ratio of Ti is 1:5; after pickling, it is washed and dried, and ultrasonically cleaned with deionized water for 10 min, and dried at room temperature for later use, abbreviated as Ti.

[0055] (2) Surface treatment: polish the titanium sheet pretreated in step (1) with 400, 800, and 1200 mesh sandpaper in sequence until the surface is smooth and free of scratches;

[0056] (3) Electrodeposition: the titanium or titanium alloy processed in step (2) is used as a working electrode, the graphite sheet is used as a counter electrode, and a saturated calomel electrode is used as a reference electrode, and electrodeposition is carried out in a solution containing dopant and Py ( Such as figure 1 shown), the obtained samples were cleaned with deionized wa...

Embodiment 2

[0058] (1) Pretreatment: Pickling the titanium sheet, during pickling, the pickling solution is composed of HF with a mass fraction of 40%, and HNO with a mass fraction of 60%. 3 Mixed, pickling time 5min; HF, HNO 3 The volume ratio is 1:4. Wash and dry, and ultrasonically clean with deionized water for 10 min, and dry at room temperature before use, abbreviated as Ti.

[0059] (2) Surface treatment: polish the titanium sheet pretreated in step (1) with 600 mesh, 1200 mesh, and 2000 mesh sandpaper in sequence until the surface is smooth without scratches for later use;

[0060] (3) Electrodeposition: the titanium or titanium alloy processed in step (2) is used as a working electrode, the graphite sheet is used as a counter electrode, and a saturated calomel electrode is used as a reference electrode, and electrodeposition is carried out in a solution containing dopant and Py, The obtained samples were cleaned successively with deionized water and ethanol, and dried in an ove...

Embodiment 3

[0062] (1) Pretreatment: carry out pickling to sample, during pickling, the pickling solution is 40% HF by mass fraction, the HNO that mass fraction is 60% 3 Mixed, pickling time is 5min; HF, HNO 3 The volume ratio is 1:6. Wash and dry, and ultrasonically clean with deionized water for 10 min, and dry at room temperature before use, abbreviated as Ti.

[0063] (2) Surface treatment: polish the titanium sheet pretreated in step (1) with 400 mesh, 1000 mesh, and 2000 mesh sandpaper in sequence until the surface is smooth without scratches for later use;

[0064] (3) Electrodeposition: the titanium or titanium alloy processed in step (2) is used as a working electrode, the graphite sheet is used as a counter electrode, and a saturated calomel electrode is used as a reference electrode, and electrodeposition is carried out in a solution containing dopant and Py, The obtained samples were cleaned successively with deionized water and ethanol, and dried in an oven; wherein, the el...

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Abstract

The invention relates to a monitoring device and a monitoring method for bacterium / cell growth. The monitoring device comprises a monitoring station and a three-electrode system, in the three-electrode system, a working electrode is a medical polypyrrole electrode, a saturated reference electrode is a calomel electrode, and a counter electrode is a platinum sheet; the test method comprises the following steps: selecting proper frequency and alternating voltage on a medical polypyrrole electrode through an alternating-current impedance technology to obtain an impedance spectrum of bacterial / cell growth, and selecting a proper analog circuit to simulate the impedance spectrum so as to achieve the purpose of monitoring the bacterial / cell growth. The monitoring method is easy to operate and low in cost.

Description

technical field [0001] The invention belongs to the field of biological monitoring, and provides a monitoring device and a monitoring method for bacteria / cell growth. Background technique [0002] In everyday life, bacterial biofilms can form on almost any surface. Bacterial growth can be beneficial on the one hand and harmful on the other. In recent years, research fields related to bacterial biofilms, especially biofilm monitoring methods, have received more and more attention. Effective biofilm detection methods are of great significance for analyzing the structural characteristics of biofilms, explaining biofilm phenomena, and solving biofilm problems. There is an urgent need for a sensor that can monitor the growth of bacterial biofilms. [0003] Traditional bacterial growth monitoring technology cannot realize real-time monitoring of bacterial / cell growth, and is destructive to bacterial / cell growth, such as using a hemocytometer to count the number of living cells ...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/30
CPCG01N27/416G01N27/301G01N27/308
Inventor 王东辉梁圣杰
Owner HEBEI UNIV OF TECH
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