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New application of gentiopicroside

A kind of technology of gentiopicroside and new use, applied in the field of new use of gentiopicroside, to achieve the effect of high biological activity and wide application prospect

Pending Publication Date: 2022-05-06
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term heavy use of the same chemical herbicides can cause serious weed resistance problems

Method used

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  • New application of gentiopicroside
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  • New application of gentiopicroside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The effect of embodiment 1 compound on the germination of spontaneous rape seeds and the growth of seedlings

[0026] 1. Liquid preparation

[0027] A certain amount of the compound Gentiopicroside was weighed, pre-dissolved in 1% (v / v) dimethyl sulfoxide (DMSO), and distilled to a concentration of 10, 25, 50, 100, 200 μg / mL with distilled water. A distilled aqueous solution containing 1% (v / v) DMSO was used as a control.

[0028] 2. Test and analysis method

[0029] The activities of different concentrations of the compound Gentiopicroside solutions on seed germination and seedling growth of self-growing rapeseed Haoyou 11 were determined by petri dish bioassay. Petri dishes (d=9 cm) were sterilized in an autoclave at 121° C. for 20 min. Soak the test seeds in 5% NaClO solution for 10 minutes for sterilization, and then wash the seeds three times with sterile water. 20 volunteer rapeseed seeds were placed in Petri dishes with filter paper respectively. Only distil...

Embodiment 2

[0036] Example 2 Effects of compounds on seed germination and seedling growth of grass weed wild oat

[0037] The significant effect of the compound on the seed germination and growth of the monocotyledonous weed wild oat (Avenafatua L.) was screened out from the aqueous solutions of different concentrations of the pure product.

[0038] 1. Pre-treatment of the tested seeds

[0039] Select wild oat seeds with the same size and full grains, peel off the seed coat, soak in 2.5% sodium hypochlorite solution, and stir with a magnetic stirrer for 10 minutes, then stir and rinse with sterile water 3 times (3 minutes each time), absorb water. Blot dry and accelerate germination for 24 hours at 25°C. The test seeds with the same dew and whiteness were selected for determination.

[0040] 2. Liquid preparation

[0041] Spread double layers of filter paper in a glass Petri dish (Ф=9cm) after autoclaving, add 5.00 mL of prepared aqueous solutions with different concentrations of 10, 2...

Embodiment 3

[0054] Example 3 Effects of compounds on seed germination and seedling growth of Gramineae brome

[0055] The significant effect of the compound on the germination and growth of the monocotyledonous weed brome brome was screened from the aqueous solutions of different concentrations of its pure product.

[0056] 1. Pre-treatment of the tested seeds

[0057] Select wild oat seeds with the same size and full grains, peel off the seed coat, soak in 2.5% sodium hypochlorite solution, and stir with a magnetic stirrer for 10 minutes, then stir and rinse with sterile water 3 times (3 minutes each time), absorb water. Blot dry and accelerate germination for 24 hours at 25°C. The test seeds with the same dew and whiteness were selected for determination.

[0058] 2. Liquid preparation

[0059] Spread double layers of filter paper in a glass Petri dish (Ф=9cm) after autoclaving, add 2.00 mL of prepared aqueous solutions with different concentrations of 10, 25, 50, 100, and 200 μg / ml ...

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Abstract

The invention discloses a novel application of gentiopicroside. The gentiopicroside is used as a herbicide. The gentiopicroside disclosed by the invention is extracted and separated from potato rhizosphere secretions in the early stage, and has an obvious inhibition effect on seed germination and seedling growth of receptor dicotyledonous weed authigenic rape and grassy weeds such as wild oats and bromegrass through a weeding biological activity test; the compound belongs to a secondary metabolite of a potato plant participating in a life activity process, has a fixed degradation path in nature, is safe and low-risk, has relatively high biological activity, and has a wide application prospect in the aspect of weeding in the later stage.

Description

technical field [0001] The invention relates to a herbicide, in particular to a new application of gentiopicroside. Background technique [0002] Weeds compete with crops for light, water, fertilizer and other resources and become an important factor affecting crop yield and quality. For a long time, chemical herbicides have been mainly used to control the damage of weeds. However, food safety, The adverse effects on public health, natural environment and weed resistance (resistance) have attracted people's attention day by day. [0003] The widespread use of chemical pesticides has played a huge role in ensuring high and stable crop yields. However, the long-term use of traditional chemical pesticides has led to the resistance of pests, forcing people to increase the dosage of pesticides, causing more and more serious environmental problems. For example, pesticide residues in soil and agricultural products are increasing year by year, endangering the safety of people and l...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P13/00
CPCA01N43/90
Inventor 李玮沈硕
Owner QINGHAI UNIVERSITY
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