The present invention discloses a pesticide composition containing picloram or picloram salt, and application of the pesticide composition containing the picloram or the picloram salt. The pesticide composition comprises effective ingredients of a component I and a composition II, wherein the component I is the picloram or the picloram salt; the composition II comprises tribenuron-methyl, bensulfuron methyl, thifensulfuron methyl, bispyribac-sodium or thiencarbazone-methyl; the mass ratio of the component I to the composition II is (1:50)-(25:1). The present invention also discloses a preparation prepared from the pesticide composition and suitable for being used in agriculture, and application of the preparation in preventing and killing of broadleaf weeds in wheat fields. The pesticide composition containing the picloram or the picloram salt has an obvious synergistic effect, reduces use amount of pesticides, the use times of the pesticides, the use cost of the pesticides, and adverse effects of the pesticides on ecological environments, enlarges weeding range, can solve a problem of various broadleaf weeds by one-time pesticide applying, makes up for defects and limitation caused by use of single agents, prolongs the service life of agents, and has important significance on comprehensive treatment of weed resistance; and the pesticide composition is fast in effect acting, long in effect period, and low in use cost, and has wide development and popularization prospects.