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Lactobacillus plantarum SF-L38 and application thereof in preparation of blood glucose control products

A technology of SF-L38 and Lactobacillus plantarum, applied in the field of microorganisms, can solve the problems of large side effects of western medicine and slow effect of traditional Chinese medicine, and achieve the effects of inhibiting the conversion of polysaccharides into monosaccharides, high adhesion, prevention and treatment of diabetes

Active Publication Date: 2022-05-06
SHANDONG SUNFLOWER BIOENGINEERING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the problems of large side effects of western medicine and slow effect of traditional Chinese medicine in the prior art, the present invention provides a kind of Lactobacillus plantarum SF-L38 and its application in the preparation of blood sugar control products, which can effectively inhibit the conversion of polysaccharides into monosaccharides in the human body and improve blood sugar indicators , has the effect of preventing and treating diabetes

Method used

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  • Lactobacillus plantarum SF-L38 and application thereof in preparation of blood glucose control products
  • Lactobacillus plantarum SF-L38 and application thereof in preparation of blood glucose control products
  • Lactobacillus plantarum SF-L38 and application thereof in preparation of blood glucose control products

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preparation example Construction

[0034] The preparation method of each substance extract of the following examples is as follows: wash ginseng, wolfberry, poria cocos, kudzu root, astragalus, mulberry, balsam pear, and Polygonatum odoratum, break them into coarse grains, and decoct them twice with 10 times the amount of water respectively , the decocting time for two times is 3h, 1h, combine the two filtrates, let stand for 2h and then filter to obtain the extract, concentrate the extract to 1.10 (80°C) under the condition of vacuum degree -0.08Mpa, then spray dry, and finally Grind it into 80 mesh fine powder to get the extract.

[0035] The preparation method of the Chinese yam powder in the following examples is as follows: wash the yam flakes with clean water, put them in boiling water for 8 minutes, remove the yam flakes and rinse off the mucus with clean water, then dry the yam flakes and process them into powder by electric grinding to obtain Chinese yam powder.

[0036] The preparation method of foll...

Embodiment 1

[0039] This example is the isolation and identification of Lactobacillus plantarum SF-L38

[0040] 1. Bacterial source: yogurt, collected in January 2021, and collected in Xining City, Qinghai Province.

[0041] 2. Isolation of strains

[0042] Do 10-fold gradient dilution of the yogurt sample, take 100 μL of each gradient and evenly spread it on the MRS solid plate medium, then take it out and place it in an anaerobic tank, and culture it overnight at 37°C;

[0043] On the next day, colonies of various shapes and sizes could be seen growing on the plate, and some characteristic colonies of lactic acid bacteria were picked for second-generation streak purification, and the plate was anaerobically activated for 1 day to obtain cultures, which were then identified for strain identification.

[0044] The Lactobacillus plantarum SF-L38 isolated in the present invention has the following microbiological characteristics: it is a round-terminus straight bacillus, usually 0.9-1.2vtm×...

Embodiment 2

[0055] The preparation method of plantarum lactobacillus SF-L38 fermented drink of the present invention comprises the following steps:

[0056] (1) First, 60 parts of purified water, 1 part of ginseng extract, 3 parts of Chinese yam powder, 3 parts of wolfberry extract, 2 parts of Poria cocos extract, 2 parts of kudzu root extract, 3 parts of astragalus extract, and 2 parts of mulberry extract , 4 parts of balsam pear extract, 2 parts of Polygonatum polygonatum extract, and 4 parts of carrot juice are mixed according to the formula ratio to obtain a mixed slurry.

[0057] (2) Transfer the mixed slurry prepared in step (1) to a sterilization container, sterilize by autoclaving at 120°C for 30 minutes, and then cool to 40°C to obtain a sterilizing solution;

[0058] (3) Inoculate Lactobacillus plantarum SF-L38 into MRS liquid medium with an inoculum size of 1%, and culture it at 37°C for 18 hours to prepare the inoculant;

[0059] (4) Inoculate the Lactobacillus plantarum SF-L...

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Abstract

The invention relates to the technical field of microorganisms, in particular to lactobacillus plantarum SF-L38 and application of the lactobacillus plantarum SF-L38 in preparation of blood glucose control products, the lactobacillus plantarum SF-L38 is preserved in China General Microbiological Culture Collection Center on September 23, 2021, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No. 23475, and the preservation number is CGMCC No. 23475. The lactobacillus plantarum is classified and named as lactobacillus plantarum. The lactobacillus plantarum SF-L38 provided by the invention has high adhesion and strong gastrointestinal adaptability, and the lactobacillus plantarum SF-L38 is used as a strain for fermentation to prepare the fermented beverage, so that polysaccharide can be inhibited from being converted into monosaccharide, the blood sugar index can be improved, and the lactobacillus plantarum SF-L38 has important functions of preventing and treating diabetes mellitus.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a plant lactobacillus SF-L38 and its application in the preparation of blood sugar control products. Background technique [0002] Diabetes is a common, multiple, and lifelong disease caused by an inappropriate increase in blood glucose (a simple sugar) level due to the inability of the body to release or utilize insulin normally. In recent years, the prevalence of diabetes in China has gradually increased. According to the survey data: "Among the population over 20 years old in my country, the prevalence of diabetes in men and women is 10.6% and 8.8% respectively, and the overall prevalence of diabetes is 9.7%. From this, the total prevalence of diabetes in the country can be calculated. The number of people with diabetes is about 92 million.” In 2019, the number of people with diabetes in China was about 116 million, and China has become the country with the largest numb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/10A23L2/38A23L33/00C12R1/25
CPCC12N1/20A61K35/747A61P3/10A23L2/382A23L33/00A23V2002/00A23V2400/169A23V2200/328
Inventor 马明磊孙浩王艳唐元凤夏越张环环陈美玉曲佳慧隋岩
Owner SHANDONG SUNFLOWER BIOENGINEERING CO LTD
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