Spodoptera frugiperda sex attractant as well as preparation method and application thereof
A technology of Spodoptera frugiperda and lure, applied in the direction of botanical equipment and methods, application, and attractant pests, can solve the problems of limited effective use period in the field, reduce the decomposition rate of sex pheromones, etc., and achieve the reduction of direct contact , Improve the slow-release time and prolong the effect of using time
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Embodiment 1
[0022] 1) Preparation of sex pheromones of fall armyworm
[0023] Spodoptera frugiperda sex pheromone (Z)-9-tetradecen-1-ol acetate, (Z)-7-dodecen-1-ol acetate according to the mass percentage of 96.5:3.5 Weigh a total amount of 30mg of the sex pheromone mixed solution of Spodoptera frugiperda, and dilute to 1mL with dichloromethane;
[0024] 2) Preparation of sex lure for Spodoptera frugiperda
[0025] Such as figure 1 As shown, take 15mL, dissolve 0.1% chitosan in 1% acetic acid, adjust the pH to 5 with sodium hydroxide solution, take the mixed solution prepared in 1) and add it under stirring, and then add 6mL, 0.1% three Add sodium polyphosphate dropwise to the above mixed solution, continue to stir at room temperature for 2 hours, and then centrifuge at 14000g for 30 minutes at 4°C to obtain the sex attractant of Spodoptera frugiperda;
[0026] 3) Preparation of sex lures for Spodoptera frugiperda
[0027] Mix the sex lure of fall armyworm obtained in step 2) with 10m...
Embodiment 2
[0029] The difference between this example and Example 1 is that: the sex lure solution of Spodoptera frugiperda obtained in step 2 was directly put into a centrifuge tube, and the other things were the same, and the sex lure core of Spodoptera frugiperda was obtained, code A2.
Embodiment 3
[0031] The difference between this embodiment and embodiment 1 is:
[0032] 1) Two different components of the sex pheromone of Spodoptera frugiperda were prepared according to the mass percentage of 96.5:3.5, the mass of the mixed solution was 30 mg, diluted with n-hexane to 1 mL, and the mixed solution of sex pheromone of Spodoptera frugiperda was obtained;
[0033] 2) Mix the sex pheromone mixture of Spodoptera frugiperda in step 1) with 30mL, 10% agar, and then quickly cool down to obtain a gelatinous solid sex attractant, and place it in a centrifuge tube to obtain Spodoptera frugiperda Moth lure lure, code A3.
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