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Cell three-dimensional rotating chip based on inductive charge electroosmosis

A technology of three-dimensional rotation and induced charge, which is applied in the field of cell analysis, can solve the problems of three-dimensional rotation operation of particles and cells, and achieve the effect of simple processing and low applied voltage

Pending Publication Date: 2022-05-10
NORTHWESTERN POLYTECHNICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of manipulation technologies for particles, cells, and multicellular organisms have been established. Among them, the manipulation technology based on electric field is the most commonly used and the easiest to integrate. However, the current existing technologies cannot realize three-dimensional rotation operations on particles and cells.

Method used

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  • Cell three-dimensional rotating chip based on inductive charge electroosmosis
  • Cell three-dimensional rotating chip based on inductive charge electroosmosis
  • Cell three-dimensional rotating chip based on inductive charge electroosmosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] See figure 1 with figure 2 , figure 1 It is a schematic structural diagram of a three-dimensional rotating cell chip based on induced charge electroosmosis provided by an embodiment of the present invention, figure 2 It is a structural schematic diagram of a first excitation electrode, a second excitation electrode and a suspension electrode provided by an embodiment of the present invention. An embodiment of the present invention provides a three-dimensional cell rotation chip based on induced charge electroosmosis. The three-dimensional cell rotation chip includes: a substrate 1, a cover sheet 2, a first excitation electrode 3, a second excitation electrode, and a suspension electrode 5, wherein,

[0033] The cover sheet 2 is arranged on the base 1, and a channel 6 passing through the cover sheet 2 is provided on the cover sheet 2 along a preset direction, wherein the preset direction is, for example, the length direction of the cover sheet 2;

[0034] The first ...

Embodiment 2

[0054] In this example, the experimental verification of the cell three-dimensional rotating microfluidic chip based on induced charge electroosmosis in the first example is carried out. See Table 1 for the size data of the first excitation electrode 3 , the second excitation electrode 4 , the suspension electrode 5 and the channel 6 of the cell three-dimensional rotation microfluidic chip based on induced charge electroosmosis in this embodiment.

[0055] During the three-dimensional rotation experiment of cells: first configure the buffer. Add a certain amount of deionized water into the beaker to obtain a buffer solution with a conductivity of 8 mS / m, and then use the buffer solution to prepare a yeast cell solution of about 100 cells / microliter.

[0056] Secondly, absolute ethanol and Tween solution were prepared at a volume ratio of 9:1 to obtain solution A. The function of solution A is mainly to reduce the adhesion of particles on the channel or the surface of the subst...

Embodiment 3

[0066] This embodiment provides a method for preparing a cell three-dimensional rotating microfluidic chip based on induced charge electroosmosis, the method comprising:

[0067] Step 1: PDMS channel processing, in this embodiment, the channel depth is 50 μm.

[0068] Specifically include the following steps:

[0069] 1) Pre-treatment of the glass substrate: first, wash it by hand with detergent, then ultrasonically clean it in acetone and isopropanol for 10 minutes, then rinse it with ionized water, and dry it with nitrogen; finally, place the dried glass substrate on In the oven, heat at 80°C for 15 minutes.

[0070] 2) Photoresist coverage: In order to obtain a channel with a depth of 50 μm, a photoresist of the type DuPont photosensitive dry film ST925 is used. Since the thickness of a single layer of photoresist is 25 μm, two layers of dry film are used here for coverage.

[0071] First, select the glass that has been cleaned, and drop a small amount of water on the sur...

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Abstract

The invention discloses a cell three-dimensional rotating chip based on inductive charge electroosmosis, which comprises a substrate, a cover plate, a first excitation electrode, a second excitation electrode and a suspension electrode, the cover plate is arranged on the substrate, and a channel penetrating through the cover plate is arranged on the cover plate along a preset direction; the first excitation electrode, the second excitation electrode and the suspension electrode are all arranged on the substrate, part of the first excitation electrode and part of the second excitation electrode are located under the channel, at least part of the suspension electrode is located under the channel, and the first excitation electrode and the second excitation electrode are symmetrically arranged along the channel; the suspension electrode is located between the first excitation electrode and the second excitation electrode. According to the cell three-dimensional rotation micro-fluidic chip based on inductive charge electroosmosis, an alternating current electric field is applied to the two excitation electrodes, ions on the double electric layers of the suspension electrodes are driven to slip to generate vortexes, and the vortexes with proper size and flow velocity are generated by changing parameters of the applied electric field to drive cells to realize three-dimensional rotation.

Description

technical field [0001] The invention belongs to the technical field of cell analysis, and relates to a three-dimensional cell rotating chip based on induced charge electroosmosis. Background technique [0002] Precise rotational manipulation of particles, cells, and multicellular organisms is a fundamental capability in biotechnology that impacts various disciplines, including single-cell analysis, drug discovery, and biological research. Rotation manipulation provides three-dimensional (3D) viewing, revealing hidden genetic, cellular, and structural details that are critical in small organism phenotyping, drug screening, and microsurgery that are invisible during typical translation manipulations arrived. Therefore, realizing the three-dimensional rotation manipulation of particles, cells and multicellular organisms is of great value in the field of biomedical research. [0003] Micro-fluidic chip (Micro-fluidic Chip) technology is widely used in single-cell analysis, med...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/42B01L3/00
CPCC12M23/16C12M45/07C12M33/04B01L3/50273B01L2400/0415
Inventor 吴玉潘张哲欣张展邓玥超鲁其怀王少熙
Owner NORTHWESTERN POLYTECHNICAL UNIV
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