Thaflavin nutrition preparation for enhancing skeletal muscle movement characteristics and application thereof
A kind of sports characteristics, theaflavin technology, applied in the fields of application, medical preparations containing active ingredients, muscular system diseases, etc.
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Embodiment 1 0
[0023] Example 1 Preparation of 60% theaflavin (purity of theaflavin detected by HPLC is 60%) mixture
[0024] Accurately weigh 200 mL of various types of macroporous resins (HP-20, SP-70), soak them in 95% ethanol for 24 h, wash with distilled water after fully swelling until the washing solution has no milky white, and wet-pack the columns (column diameter-to-height ratio =5:15), washed with water until neutral, set aside.
[0025] The specific method of use is to use different washing methods for different types of macroporous resins; when using HP-20 macroporous resins to separate theaflavins, use 75% ethanol gradient elution, collect the solution that has been eluted for 1 hour, and freeze-dry To be tested; SP-70, after elution with 50% ethanol for half an hour, change to 75% ethanol to continue elution, collect the solution at 45mins, freeze-dry for testing.
[0026]The final optimized process is, black tea: water = 2:1, after soaking in hot water for 15 minutes, filter...
Embodiment 2
[0029] Example 2 Evaluation of the Effect of Regulating Glucose Absorption and Mitochondria Generation in Skeletal Muscle
[0030] Resveratrol has been widely reported to have good biological functions on skeletal muscle development and mitochondrial development. The resveratrol used in the experiment was purchased from Hunan Sanfu Biotechnology Co., Ltd., and the content detected by high performance liquid chromatography was 98%. Eucommia is a traditional medicine that nourishes the liver and kidney, and strengthens bones and muscles. The specific preparation method of Eucommia ulmoides extract in this experiment is as follows: add 10 times the amount of eucommia leaves to reflux for extraction for 1 hour, collect the extract, separate it on a polyamide column, elute with 3BV50% ethanol, and recover the ethanol from the eluent and then dry it in vacuum to obtain The required extract of Eucommia ulmoides, the content of chlorogenic acid detected by high performance liquid chro...
Embodiment 3
[0031] Example 3 Evaluation of Effects of Regulating Glucose Absorption and Mitochondria Generation in Skeletal Muscle
[0032] Using C2C12 myoblasts as a model, after inducing differentiation into mature myotubes in vitro, the mature myotubes were incubated with LPS (40 mg / ml) for 24 hours to construct a myotube inflammation model.
[0033] Then use the formula in Example 2 to treat the inflammatory myotubes, use Q-PCR, ELISA, and atomic force electron microscopy to detect the inflammation level and mechanical properties of the myotubes, and clearly determine the specific effect of the formula of the present invention on myotube inflammation.
[0034] The specific experimental process is as follows:
[0035] 1. Configuration of various medium for cell culture
[0036] Cell culture medium: ① Proliferation medium (GM): 10% fetal bovine serum + DMEM high-glucose medium; ② Differentiation medium (DM): 2% horse serum + DMEM high-glucose medium; ③ 60% tea Flavin (20 μg / ml) + Euco...
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