Bioactive peptide prepared from snails as well as preparation method and application of bioactive peptide
A technology of bioactive peptides and snails, which is applied in the field of active peptides to achieve the effects of good flavor, light color, and improved taste and aroma
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Embodiment 1
[0047] This embodiment provides a method for preparing biologically active peptides from snail protein, which specifically includes the following steps:
[0048] S1. Processing of snails: wash the snails, remove the shells, and separate the viscera and snail meat;
[0049] S2. Preparation of helicase: add phosphate buffer solution with a pH value of 5.5 to the viscera obtained in step S1, the mass volume ratio of the viscera to buffer is 1:3g / mL, pulverize with a meat grinder, and centrifuge at 3000r / min for 15min , taking the supernatant, using an ultrafiltration membrane with a pore size of 1000D to concentrate to a relative water density of 2, and freeze-drying to obtain helicase;
[0050] S3. Preparation of minced snail meat: crushing the snail meat with a meat grinder to obtain minced snail meat;
[0051] S4. Snail enzyme enzymolysis: Add 75 parts by weight of water and 2 parts by weight of helicase obtained in step S2 to 50 parts by weight of the minced snail meat obtai...
Embodiment 2
[0059] This embodiment provides a method for preparing biologically active peptides from snail protein, which specifically includes the following steps:
[0060] S1. Processing of snails: wash the snails, remove the shells, and separate the viscera and snail meat;
[0061] S2. Preparation of helicase: Add phosphate buffer solution with a pH value of 6 to the viscera obtained in step S1, the mass volume ratio of the viscera to the buffer solution is 1:5g / mL, pulverize with a meat grinder, and centrifuge at 3000r / min for 15min , taking the supernatant, using an ultrafiltration membrane with a pore size of 2000D to concentrate to a relative water density of 4, and freeze-drying to obtain helicase;
[0062] S3. Preparation of minced snail meat: crushing the snail meat with a meat grinder to obtain minced snail meat;
[0063] S4. Helicase enzymolysis: Add 100 parts by weight of water and 5 parts by weight of helicase prepared in step S2 to 50 parts by weight of the minced snail me...
Embodiment 3
[0071] This embodiment provides a method for preparing biologically active peptides from snail protein, which specifically includes the following steps:
[0072] S1. Processing of snails: wash the snails, remove the shells, and separate the viscera and snail meat;
[0073] S2. Preparation of helicase: add phosphate buffer solution with a pH value of 5.7 to the viscera obtained in step S1, the mass volume ratio of the viscera to the buffer solution is 1:4g / mL, pulverize with a meat grinder, and centrifuge at 3000r / min for 15min , taking the supernatant, using an ultrafiltration membrane with a pore size of 1500D to concentrate to a relative water density of 3, and freeze-drying to obtain helicase;
[0074] S3. Preparation of minced snail meat: crushing the snail meat with a meat grinder to obtain minced snail meat;
[0075] S4. Helicase enzymolysis: Add 85 parts by weight of water and 3.5 parts by weight of helicase prepared in step S2 to 50 parts by weight of the minced snail...
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