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Establishment method of UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum of rhizoma gastrodiae vertigo

A technology of fingerprint spectrum and establishment method, which is applied to the establishment method of UPLC fingerprint spectrum of Tianma Xuanduanning granules and the field of fingerprint spectrum, and achieves the effects of good peak shape, overcoming single detection index and comprehensive preparation quality.

Pending Publication Date: 2022-05-13
LUNAN HOPE PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no patent publications or literature reports on the fingerprint analysis of Gastrodia elata vertigo granules by UPLC method

Method used

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  • Establishment method of UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum of rhizoma gastrodiae vertigo
  • Establishment method of UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum of rhizoma gastrodiae vertigo
  • Establishment method of UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum of rhizoma gastrodiae vertigo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Establishment of UPLC Standard Fingerprint of Gastrodia Tianma Vertigo Ning Granules

[0044] 1 Instruments and reagents

[0045] 1.1 Instrument

[0046] Waters AcquityArc high-performance liquid chromatography (USA): 2998PDA detector, quaternary ultra-high pressure gradient pump, Empower chromatography workstation.

[0047] 1.2 Reagent

[0048] Tianma Zhenyunning Granules were prepared by Lunan Houpu Pharmaceutical Co., Ltd. according to the above method, and the sample batch numbers are shown in Table 1. Acetonitrile was chromatographically pure, water was double distilled water, and other reagents were analytically pure.

[0049] Table 1 Batch number of test samples of Gastrodia Tianma Vertigo Ning Granules

[0050]

[0051] 2 Methods and results

[0052] 2.1 Chromatographic conditions: Chromatographic column: Agilent Eclipse Plus C 18 Column, 4.6×150mm, particle size 5μm; acetonitrile as mobile phase A, 0.10% phosphoric acid solution as mobile pha...

Embodiment 2

[0076] Example 2 Establishment of UPLC Standard Fingerprint of Gastrodia Tianma Vertigo Ning Granules

[0077] 1 Instruments and reagents

[0078] 1.1 Instrument

[0079] Waters AcquityArc high-performance liquid chromatography (USA): 2998PDA detector, quaternary ultra-high pressure gradient pump, Empower chromatography workstation.

[0080] 1.2 Reagent

[0081] Tianma Vertigo Ning Granules were provided by Lunan Houpu Pharmaceutical Co., Ltd., and the sample batch numbers are shown in Table 1. Acetonitrile was chromatographically pure, water was double distilled water, and other reagents were analytically pure.

[0082] 2 Methods and results

[0083] 2.1 Chromatographic conditions: Chromatographic column: Agilent Eclipse Plus C 18 Column, 4.6×150mm, particle size 5μm; acetonitrile as mobile phase A, 0.15% phosphoric acid solution as mobile phase B, eluted by gradient change of mobile phase content and flow rate; detection wavelength: 210nm; column temperature: 33°C; inje...

Embodiment 3

[0092] Example 3 Establishment of UPLC Standard Fingerprint of Gastrodia Tianma Vertigo Ning Granules

[0093] 1 Instruments and reagents

[0094] 1.1 Instrument

[0095] Waters AcquityArc high-performance liquid chromatography (USA): 2998PDA detector, quaternary ultra-high pressure gradient pump, Empower chromatography workstation.

[0096] 1.2 Reagent

[0097] Tianma Vertigo Ning Granules were provided by Lunan Houpu Pharmaceutical Co., Ltd., and the sample batch numbers are shown in Table 1. Acetonitrile was chromatographically pure, water was double distilled water, and other reagents were analytically pure.

[0098] 2 Methods and results

[0099] 2.1 Chromatographic conditions: Chromatographic column: Agilent Eclipse Plus C 18 Column, 4.6×150mm, particle size 5μm; acetonitrile as mobile phase A, 0.28% phosphoric acid solution as mobile phase B, eluted by gradient change of mobile phase content and flow rate; detection wavelength: 254nm; column temperature: 37°C; inje...

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Abstract

The invention belongs to the field of analysis of traditional Chinese medicine preparations, and particularly discloses a method for establishing a UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum of gastrodia elata Jiangning granules, which comprises the following steps: preparing a gastrodia elata Jiangning granule reference substance and a test solution, determining UPLC chromatographic conditions and manufacturing a UPLC standard fingerprint spectrum. The invention also discloses the UPLC standard fingerprint spectrum of the gastrodia elata Jiangning granules obtained by the method, and the obtained fingerprint spectrum has 31 common peaks. The method is easy and convenient to operate, high in stability and good in reproducibility, the obtained fingerprint spectrum has many common peaks and good separation degree, the quality of the gastrodia elata Jiangning granules can be comprehensively evaluated and controlled by comparing the common peaks with common peaks in a standard fingerprint spectrum, it is ensured that the internal quality of the gastrodia elata Jiangning granules is uniform and stable, and the quality of the gastrodia elata Jiangning granules is improved. And the safety and effectiveness of clinical medication are improved.

Description

technical field [0001] The invention belongs to the field of analysis of traditional Chinese medicine preparations, and in particular relates to a method for establishing the UPLC fingerprint of Gastrodia elata vertigo and its fingerprint. Background technique [0002] The prescription and preparation of Tianma Zhenyunning Mixture are recorded in Volume 14 of the Ministry of Health Drug Standards for Traditional Chinese Medicine Prescriptions (No. WS3-B-2661-97). The same series products of Gastrodia elata vertigo, including Gastrodia elata elixir, Gastrodia elixir granules, etc., are classic prescriptions for the treatment of vertigo. It is made of 12 raw materials such as tangerine peel and ginger. It has the effects of eliminating phlegm, calming dizziness, harmonizing the stomach and relieving vomiting. It is mainly used clinically for dizziness, nausea, vomiting, pale tongue, white and slippery coating, especially for Meniere's disease (Ménière's disease), labyrinthiti...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634G01N30/8686Y02A50/30
Inventor 关永霞孙晓兰徐丽庄会芳
Owner LUNAN HOPE PHARM CO LTD
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