Near-infrared two-region microscopic imaging system and method based on laser scanning

A technology of microscopic imaging and laser scanning, which is applied in the field of bioluminescent imaging to achieve the effects of increased scanning efficiency and speed, improved time resolution, improved compatibility and flexibility

Pending Publication Date: 2022-05-24
广州市凯佳光学科技有限公司
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  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a laser scanning-based near-infrared two-zone microscopic imaging system and method, which can solve at least one of the technical problems of bioluminescence imaging in the prior art

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  • Near-infrared two-region microscopic imaging system and method based on laser scanning
  • Near-infrared two-region microscopic imaging system and method based on laser scanning

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Embodiment Construction

[0045]The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0046] Unless expressly stated otherwise, throughout the specification and claims, the term "comprising" or its conjugations such as "comprising" or "comprising" and the like will be understood to include the stated elements or components, and Other elements or other components are not excluded.

[0047] like figure 1 As shown in the figure, an embodiment of the present invention provides a near-infrared two-zone microscopic imaging system based on laser scanning, including: a multi-wavelength laser module, a beam shaping module, a multi-focus structured light generation module, a beam relay and scanning module, an imaging module with the detection module.

[0048] Among them, the multi-wavelength laser module is used t...

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Abstract

The invention belongs to the technical field of biological fluorescence imaging, and provides a near-infrared two-region microscopic imaging system and method based on laser scanning, and the method comprises the steps: carrying out the beam deflection and beam combination of laser beams with different wavelengths, and obtaining combined light; shaping the combined light to obtain shaped light; phase-modulating the shaped light to generate a multi-focus illumination light field; relaying and two-dimensional scanning are carried out on the shaped light subjected to phase modulation, so that a multi-focus illumination light field is subjected to two-dimensional scanning on a focal plane of a microobjective, a sample is excited to emit a fluorescence signal of a near-infrared second-area wave band, and a near-infrared camera is used for detecting the fluorescence signal and imaging. According to the scheme, an optical path system is simple to integrate, and deeper imaging can be realized due to longer excitation wavelength; and the dye selection of the near-infrared second region is more flexible.

Description

technical field [0001] The invention belongs to the technical field of biological fluorescence imaging, in particular to a near-infrared second-region microscopic imaging system and method based on laser scanning. Background technique [0002] As an indispensable technical means in the field of biomedicine, biofluorescence imaging technology has been widely used, and it is a powerful tool to observe the morphology, structure and life phenomena of biological tissues. At present, the commonly used biofluorescence imaging technology is the laser scanning confocal microscope developed in the 1980s. It is characterized by the use of pinhole technology to eliminate the interference of light signals outside the focus on the image, thereby greatly improving the clarity of the image. Detail resolution with high axial contrast. Since the laser range used by the laser scanning confocal microscope is between 488nm and 647nm, it belongs to the visible light category, and biological tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/6428G01N21/6458G01N21/6486G01N21/645G01N21/01G01N2021/6463G01N2021/0112
Inventor 娄凯冯亮
Owner 广州市凯佳光学科技有限公司
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