Complex microbial inoculant culture device and method
A technology of compound bacterial agent and culturing device, which is applied in sterilization methods, biochemical cleaning devices, microorganism-based methods, etc., can solve the problems of inability to co-exist and co-prosper, difficult to exert, and cannot achieve expected goals, and achieves improvement. The effect of soil quality, ecological restoration and ingenious structure
Pending Publication Date: 2022-05-27
SICHUAN QINGHE TECH
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Problems solved by technology
However, a single photosynthetic bacterial agent is not easy to colonize and survive in the application environment in practical applications, and it is difficult to exert obvious expected effects, while the compound bacterial agent is often complex due to the complexity of strain screening, strain compatibility, medium selection and culture conditions. The population structure is unstable, the mutual synergies of the strains are not obvious, and even antagonize each other, compete and inhibit each other, unable to coexist and prosper, and promote mutual growth, it is difficult to form a stable micro-ecological system, and the expected goal cannot be achieved
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Embodiment 2
[0046] like Figure 8 As shown, the present embodiment provides a compound bacterial agent culture method, comprising the following steps:
[0047] Culture liquid: such as Figure 7 As shown, the four compound bacterial agent culture devices in Example 1 were used to cultivate photosynthetic bacteria, nitrogen-fixing bacteria, lactic acid bacteria and yeast bacteria, respectively, and propagated to prepare bacterial liquid.
[0048] The specific operation is: put one of the photosynthetic bacteria, nitrogen-fixing bacteria, lactic acid bacteria and yeast strains, ingredients, and distilled water into the culture tank 1 from the feeding port 11, and close the feeding port 11. At this time, the roller 231 is located in the horizontal area 241 and the inclined area. At the junction of 242, in the process of culturing the bacterial liquid, mix evenly, and adjust the appropriate temperature, brightness, humidity and other parameters, and set the incubation time;
[0049] After cu...
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The invention discloses a complex microbial inoculant culture device and method. The culture device comprises a culture tank and an adjusting mechanism. The top of the culture tank is provided with a feed port and a ventilation port, and the bottom is provided with a discharge port; the adjusting mechanism comprises an adjusting plate, a clamping plate, a rectangular frame and a moving block, the clamping plate is sequentially provided with an adjusting plate groove, a rectangular frame groove and a moving block groove, the adjusting plate is arranged in the adjusting plate groove, the rectangular frame is arranged in the rectangular frame groove, and the moving block is arranged in the moving block groove; the left wall of the rectangular frame is connected with a clamping plate through a first spring, the right wall of the rectangular frame is provided with a ventilation conversion plate, the top of the moving block is provided with a horizontal area and an inclined area, the bottom of the moving block is provided with a push rod, and the push rod penetrates through the bottom of the clamping plate and then is connected with a sealing plate. And the other end of the sealing plate is positioned at the discharge hole. Bacteria such as lactic acid bacteria, photosynthetic bacteria, saccharomycetes and nitrogen-fixing bacteria can be simultaneously subjected to mixed symbiotic culture, mass proliferation is realized, and the strain activity is kept.
Description
technical field [0001] The invention relates to the technical field of compound inoculum culture, in particular to a compound inoculum culture device and method. Background technique [0002] Microbial inoculants are commonly used and most effective green ecological products in agricultural soil improvement, ecological environment management and aquaculture synergy. Among them, photosynthetic bacteria are one of the important bacterial species. However, it is difficult for a single photosynthetic bacterial inoculant to colonize and survive in the application environment in practical applications, and it is difficult to exert obvious expected effects. The population structure is unstable, the synergistic effect of strains is not obvious, and even antagonistic competition and inhibition of each other, can not symbiotic and co-prosperity, mutual promotion, it is difficult to form a stable micro-ecosystem, can not achieve the expected goals. SUMMARY OF THE INVENTION [0003] ...
Claims
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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12M1/00C12N1/20C12N1/16C12R1/01C12R1/645C12R1/225C12R1/065
CPCC12M29/06C12M29/20C12M37/04C12M41/06C12M41/12C12M41/34C12N1/20C12N1/16Y02W30/40
Inventor 肖奎简龙骥向文良宋菲菲刘学阳郑进
Owner SICHUAN QINGHE TECH