Preparation method of marine fish biological peptide
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A marine fish and bio-peptide technology, which is applied to peptide preparation methods, chemical instruments and methods, and peptides, can solve problems such as single product types, difficult industrialization, and the quality of marine fish protein peptide products cannot meet market requirements. Effect of reducing purine content
Pending Publication Date: 2022-05-27
JIANGSU ZHIHUI BIOTECH
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[0003] At present, fish protein peptides on the market are basically fish collagen peptides prepared from fish skin, fish scales, and fish bones. They focus on beauty and skin care functions, and the product types are single. There have always been technical bottlenecks in the removal and color problems, so the quality of the prepared marine fish protein peptide products cannot meet the existing market requirements, making it difficult to industrialize
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Embodiment 1
[0019] The present embodiment is to provide a preparation method of marine fish biological peptide, comprising the following steps:
[0020] 1) Peel the skin and bones of the marine fish to get the fish meat, wash the fish meat with 0-4 ℃ water, grind the fish meat, add water, and fully stir to obtain a fish slurry;
[0021] Cook for 25 minutes at a temperature of 120-125°C and a pressure of 0.8-1.5kg / cm; cool to remove grease and impurities in the upper layer to obtain a homogenate;
[0022] 2) using disodium hydrogen citrate as a buffer to adjust the pH of the above homogenate to 6-7, adding protease and an enzyme activator, the mass ratio of the homogenate, protease and enzyme activator is 100:3:0.4, Placed at 50 °C for enzymatic hydrolysis to inactivate the enzyme to obtain an enzymatic hydrolysis solution;
[0023] 3) Microfiltration of the enzymatic hydrolysate through a 45-50nm ceramic membrane, followed by a 10kDa organic ultrafiltration membrane for fractional purifi...
Embodiment 2
[0028] The present embodiment is to provide a preparation method of marine fish biological peptide, comprising the following steps:
[0029] 1) After taking the clean fish meat and draining the water, the fish meat is shredded and then added with water, and fully stirred to obtain a fish slurry;
[0030] Cook for 30 minutes at a temperature of 116-123°C and a pressure of 1-1.5kg / cm; after cooling, remove the grease and impurities in the upper layer to obtain a homogenate;
[0031] 2) utilize the mixture of trisodium citrate and dipotassium hydrogen citrate as buffer to adjust the pH of above-mentioned homogenate to 6-7, add protease and enzyme activator, the mass ratio of described homogenate, protease and enzyme activator It is 100:3.5:0.35, placed at 50 °C for enzymatic hydrolysis, inactivating the enzymatic activity, and obtaining an enzymatic hydrolysis solution;
[0032] 3) Microfiltration of the enzymatic hydrolysate through a 45-50nm ceramic membrane, followed by a 10k...
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Abstract
The invention discloses a preparation method of marine fish biological peptide, which comprises the following steps: 1) taking fresh marine fish meat, and adding water to prepare fish slurry; cooking for 20-30 minutes, and cooling to obtain homogenate; 2) adjusting the pH value of the homogenate to 5-7, adding protease and an enzyme activator, carrying out enzymolysis at 48-52 DEG C, and carrying out enzyme deactivation to obtain an enzymatic hydrolysate; 3) performing microfiltration on the enzymatic hydrolysate through a ceramic membrane, performing graded purification through an organic ultrafiltration membrane, and performing nanofiltration through a nanofiltration membrane; 4) drying the filtrate obtained in the step 3) by a freeze-drying method to obtain primary marine fish biological peptide; and 5) removing bitterness and fishy smell of the preliminary marine biological peptide prepared in the step 4). The invention belongs to the technical field of preparation of marine fish oligopeptides, and particularly relates to a preparation method of a marine fish biological peptide which is used for removing bitterness and fishy smell of the marine fish biological peptide and improving the taste, the obtained marine fish biological peptide is stable in property and high in active ingredient content, and meanwhile, the preparation method is simple in technological process, easy to operate and low in cost.
Description
technical field [0001] The invention belongs to the technical field of plant deodorants, and in particular relates to a preparation method of marine fish biological peptides. Background technique [0002] Sea fish is rich in resources, high in nutritional value and delicious in taste. It is a precious product on the table. Marine fish protein peptide is a kind of pure natural nutrients extracted from marine fish. The relative molecular mass is below 5000Da, which is easy to absorb and has a very high absorption efficiency. It not only has high nutritional value, but also has fast absorption and utilization. It has many advantages such as compatibility with other food ingredients, and has various biological activities such as antioxidant, antibacterial, blood pressure lowering, antitumor, etc., and is a good functional food ingredient. [0003] At present, the fish protein peptides on the market are basically fish collagen peptides prepared from fish skin, fish scales and fi...
Claims
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Application Information
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