Temperature-sensitive hydrogel loaded with umbilical cord mesenchymal stem cells and preparation method of temperature-sensitive hydrogel

Abstract

The invention provides a preparation method of temperature-sensitive hydrogel loaded with mesenchymal stem cells, which comprises the following specific processes: separating and iteratively culturing umbilical cord mesenchymal stem cells to obtain qualified cell bank cells, loading the obtained cell bank cells on in-situ temperature-sensitive biomedical hydrogel, and drying to obtain the temperature-sensitive hydrogel loaded with the umbilical cord mesenchymal stem cells. The final cell concentration in the temperature-sensitive hydrogel is 3 * 10 < 6 > cells / mL. According to the invention, a novel hydrogel preparation is prepared by adopting a biological macromolecular and non-free radical crosslinking mechanism of temperature-sensitive hydrogel; and screening the temperature-sensitive in-situ gel prescription and process by taking the compatibility to the stem cells and the phase transition temperature as indexes. The mesenchymal stem cells are innovatively combined with the hydrogel to prepare a novel hydrogel preparation, so that the retention of the umbilical cord mesenchymal stem cells at a wound part is realized, and the loss repair effect of the mesenchymal stem cells is better played.

Description

technical field [0001] The invention relates to a temperature-sensitive hydrogel loaded with umbilical cord mesenchymal stem cells and a preparation method thereof. Background technique [0002] Intrauterine adhesions (IUA) are due to uterine surgery, infection and other factors that lead to damage to the basal layer of the endometrium, partial or complete occlusion of the uterine cavity, and then cause symptoms such as oligomenorrhea, amenorrhea, infertility or recurrent miscarriage. Gynecological diseases seriously affect women's reproductive health. [0003] At present, the preferred method for clinical treatment of IUA is hysteroscopic adhesiolysis (TCRA). After TCRA, there is a separation wound in the uterine cavity, which is prone to the risk of re-adhesion, and the incidence rate is 62.5%. Therefore, it is clinically proposed to use the intrauterine barrier to isolate the wound and prevent re-adhesion. The intrauterine barriers currently used clinically after TCRA ...

AI Technical Summary

Problems solved by technology

[0004] IUD has been used for many years to prevent recurrence after separation of intrauterine adhesions, but IUD has the following disadvantages: copper-containing IUD can aggravate the inflammation of the separation surface, the contact area is small, it can cause abnormal uterine bleeding, infection, and severe cases can lead to Uterine perforation

Since the uterine cavity and vagina are connected, the injected stem cells may flow out, which will affect the therapeutic effect

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