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Preparation method of cryptocaryon irritans vaccine based on tetrahymena thermophila tubulin protein

A technology for stimulating Cryptocaryonia and Tetrahymena thermophila, which is applied in the field of preparation of vaccines for stimulating Cryptocaryonia, which can solve the problems of poor immune protection effect and high preparation cost of Cryptocaryonium stimulating vaccines

Pending Publication Date: 2022-06-03
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0005] The present invention aims to solve the technical problems of high preparation cost and poor immune protection effect of Cryptocaryon vaccine, and provides a preparation method for stimulating Cryptocaryon vaccine based on Tetrahymena thermophila tubulin protein. The cross-protective antigen tubulin protein in hymenophora, and the recombinant expression of tubulin gene in Escherichia coli, which is used as antigen to prepare vaccines, greatly improves the grouper's ability to resist the stimulation of Cryptocaryon infection

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  • Preparation method of cryptocaryon irritans vaccine based on tetrahymena thermophila tubulin protein

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Embodiment Construction

[0059] A kind of preparation method of the stimulated Cryptocaryon vaccine based on Tetrahymena thermophila tubulin protein, it comprises the steps:

[0060] The identification of cross-antigens

[0061] 1. Stimulate the passage of Cryptonucleus

[0062] The oval pomfret was temporarily raised in a 1000L seawater pond, and commercial feed was fed at 8:00 am and 5:00 pm, respectively; in the evening, the stimulated Cryptocaryon larvae hatched within 2 hours were added to infect the oval pomfret, stimulated The infection concentration of Cryptonuclearia was 20,000 larvae / tail fish, and the water level was controlled at 40L / tail fish during infection, and it was temporarily cultured in still water. It can be observed that there are a large number of white spots on the body surface and gills of the oval pomfret, and then the oval pomfret is transferred to a specific stimulus to the Cryptocaryon worm collector; the next morning, collect the fixed on the bottom of the worm collecto...

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Abstract

The invention aims to solve the technical problems of high preparation cost and poor immune protection effect of a cryptocaryon irritans vaccine, and provides a preparation method of a tetrahymena thermophila tubulin protein-based cryptocaryon irritans vaccine, the cross-protective antigen tubulin protein in tetrahymena is identified for the first time, and the tubulin gene is subjected to recombinant expression in escherichia coli to obtain the cryptocaryon irritans vaccine based on the tetrahymena thermophila tubulin protein. The vaccine is prepared by taking the antigen as an antigen, so that the capability of resisting cryptocaryon irritans infection of the grouper is greatly improved. And the immune protection effect of the vaccine is far higher than that of a heterologous vaccine prepared from the scorpion protein or cilia protein of tetrahymena.

Description

technical field [0001] The invention belongs to the field of preparation of a vaccine of Cryptonaria stimuli, in particular to a preparation method of a vaccine of stimulatory Cryptonariae based on Tetrahymena thermophila tubulin protein. Background technique [0002] C. stimuli is a lethal parasitic disease caused by a marine ciliate, C. stimuli, and is listed as a second-class disease of aquatic animals in the National List of Category I, II, and III Animal Diseases. In recent years, stimuli cryptocartosis has caused serious harm to marine fish in my country, causing economic losses of more than 1 billion yuan each year to my country, which greatly restricted the healthy development of marine aquaculture. Intraperitoneal injection of inactivated C. stimuli can induce immune protection against C. stimuli in fish, indicating that vaccination is an effective method to prevent and treat C. stimuli (Yoshinaga et al, 1997; Luo et al, 2007 ; Hatanaka and Hirazawa, 2008). Howeve...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/002A61K39/39C07K14/44C12N15/70A61P33/14C12R1/19
CPCA61K39/002A61K39/39A61P33/14C07K14/44C12N15/70A61K2039/55A61K2039/55566A61K2039/545C12N2800/22Y02A40/81
Inventor 李言伟但学明莫泽权吴辉城胡颖彤
Owner SOUTH CHINA AGRI UNIV
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