Application of acetoin to improvement of plant rhizosphere colonization of rhizosphere growth-promoting bacteria
A technology of rhizosphere growth-promoting bacteria and acetoin is applied in the field of microorganisms to achieve the effects of promoting plant growth, wide application prospects and increasing swimming effect
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Embodiment 1
[0026] Example 1 The effect of different concentrations of acetoin on the biofilm formation of rhizosphere growth-promoting bacteria NRCB002, NRCB005, Lv5A and NRCB010.
[0027] (1) Experimental method
[0028]Take each bacteria from the plate into a 50ml centrifuge tube, culture at 30°C and 200rpm for 12 hours, draw 1% of the inoculum into a 250 conical flask containing 50ml of LB broth, and culture overnight. Pipette 20ml into a sterilized 50ml centrifuge tube, centrifuge at 5000rpm for 5min, pour off the supernatant after centrifugation, add an equal amount of sterile normal saline and mix gently. Repeat the same operation twice. Dilute with K's medium to an OD value of about 1.0. 37.5 μL of bacterial liquid and 112.5 μL of K’s medium containing different concentrations of acetoin (0.1, 0.5, 2, 5, 10 g / L) were sequentially added to the 96-well plastic culture plate, and 8 parallels were set for each treatment, with different concentrations. The K's medium containing aceto...
Embodiment 2
[0035] Example 2 The effect of acetoin on the running diameter of rhizosphere growth-promoting bacteria NRCB002, NRCB005 and NRCB010.
[0036] According to the results obtained in Experiment 1, the effect of acetoin on the migration diameter of rhizosphere growth-promoting bacteria NRCB002, NRCB005 and NRCB010 was further explored.
[0037] (1) Experimental method
[0038] Swimming medium: Each 1L contains tryptone 10g, sodium chloride 10g, yeast powder 5g and agar powder 5g.
[0039] Use NBNS liquid medium to inoculate overnight activated strains NRCB002, NRCB005, NRCB010 seed solution. Cultured to mid-logarithmic phase (OD) at 30°C and 200rpm. 600 = 1.0). Add 100 μl of 0.1g / L acetoin solution to the Swimming plate and spread it evenly, place a circular sterile paper with a diameter of 0.5 cm in the center of the plate, and use a pipette tip to suck 5 μl of bacterial solution and slowly drop it to the center of the paper. In a sterile environment for ten minutes, the plat...
Embodiment 3
[0045] Example 3 The effect of acetoin on the colonization of rhizosphere growth-promoting bacteria NRCB002 in plant rhizosphere.
[0046] Because biofilm formation ability and swimming ability are important indicators to improve the colonization of growth-promoting bacteria in the rhizosphere, the rhizosphere growth-promoting bacteria NRCB002 was selected for Example 1 and Example 2 to study the effect of acetoin on the rhizosphere-promoting bacteria. The role of NRCB002 in rhizosphere colonization, the specific experimental methods and experimental results are as follows:
[0047] (1) Experimental method
[0048] Tomato and lettuce seed treatment: Treat with 2.5% 84 disinfectant for 10 minutes (shaking vigorously), rinse with sterile water for 7-10 times, then soak in sterile water for 5 minutes, 2 times, after removing the water, spread the seeds evenly on the The prepared agar plates were placed in a lighted incubator for germination.
[0049] Strain treatment: NRCB002 w...
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